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  • Evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates

    Creator:
    Nelson, Kimberly J., Parsonage, Derek, Karplus, P. Andrew, and Poole, Leslie B.
    Abstract:
    Peroxiredoxins (Prxs) are very effective peroxide reducing enzymes, but also are susceptible to being oxidatively inactivated by their own substrates. The level of sensitivity to such hyperoxidation varies depending both on the enzyme involved and the type of peroxide substrate. For some Prxs, the hyperoxidation has physiological relevance, so it...
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    This work was supported by U. S. Public Health Service Grant GM050389 from the National Institutes of

  • Peroxiredoxins in Parasites

    Creator:
    Gretes, Michael C., Poole, Leslie B., and Karplus, P. Andrew
    Abstract:
    Significance: Parasite survival and virulence relies on effective defenses against reactive oxygen and nitrogen species produced by the host immune system. Peroxiredoxins (Prxs) are ubiquitous enzymes now thought to be central to such defenses and, as such, have potential value as drug targets and vaccine antigens. Recent Advances: Plasmodial and...
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    - ducing agent of ROOH, ONOO- , and lipid peroxides in the cell (263), a view supported by a network model

  • Tuning of Peroxiredoxin Catalysis for Various Physiological Roles

    Creator:
    Perkins, Arden, Poole, Leslie B., and Karplus, P. Andrew
    Abstract:
    Peroxiredoxins (Prxs) make up an ancient family of enzymes that are the predominant peroxidases for nearly all organisms and play essential roles in reducing hydrogen peroxide, organic hydroperoxides, and peroxynitrite. Even between distantly related organisms, the core protein fold and key catalytic residues related to its cysteine-based catalytic mechanism have...
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    @science.oregonstate.edu 8 9 †This study was supported in part by National Institutes of Health grant RO1 GM050389 to

  • Structural studies of the antioxidant defense enzymes; copper, zinc superoxide dismutase and alkyl hydroperoxide reductase flavoprotein

    Creator:
    Roberts, Blaine R.
    Abstract:
    Oxygen derived radicals are involved in many aspects of life from aging and cell signaling to disease states as diverse as heart disease, diabetes, neurodegeneration and inflammation. Therefore, understanding the function of antioxidant defense proteins and the effects of oxygen derived radicals on protein function is essential to elucidate the...
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    Dissertation
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    in chapter 3. Leslie B. Poole and was also involved in the writing of chapter 3. Chris Holguin and

  • Biophysical analyses of peroxiredoxins and a partner reductase

    Creator:
    Hall, Andrea Renée
    Abstract:
    Peroxiredoxins (Prxs) are dominant peroxide-reducing enzymes with two important roles: they protect all organisms from oxidative damage induced by peroxides, and in eukaryotes, they participate in hydrogen peroxide signaling pathways. This dissertation presents studies aimed at the biophysical characterization of select Prxs and a Prx reductase to elucidate their structure-function...
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    you. I would like to acknowledge the National Institutes of Health for providing the funding that

  • Model systems for structural investigations into peroxiredoxin catalysis, conformation change, and inactivation

    Creator:
    Perkins, Arden
    Abstract:
    Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide, peroxynitrous acid, and organic peroxides, and are extremely efficient peroxidases, with k[subscript cat]/K[subscript M] on the order of 10⁷ - 10⁸ M⁻¹ s⁻¹. Besides their role in oxidative stress defense, evidence has accumulated that some eukaryotes, including humans, use Prxs as...
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    to human health is underscored by the occurrence of cancer in some Prx knockout mice; the

  • Functional, structural and evolutionary analyses of the ferritin-like superfamily of proteins

    Creator:
    Cooley, Richard Bleier
    Abstract:
    The ferritin-like superfamily (FLSF) of proteins is composed of a wide variety of functionally diverse proteins involved in oxygen dependent metal-mediated electron transfer reactions. Their biological importance is exemplified by the fact FLSF proteins are found in almost every organism from all three domains of life. Their functions range from...
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    home-cooked food. I would like to acknowledge the National Institutes of Health grant numbers

  • A Primer on Peroxiredoxin Biochemistry

    Creator:
    Karplus, P. Andrew
    Abstract:
    Peroxiredoxins (Prxs) were not recognized as a family of enzymes until the 1990s but are now known to be the dominant peroxidases in most organisms. Here, the history and fundamental properties of peroxiredoxins are briefly reviewed, with a special focus on describing how an exquisitely tunable balance between fully folded...
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    was supported in part by National Institutes of Health grant R01 GM50389 to Leslie B. Poole and P.A.K

  • Hydrogen/deuterium exchange mass spectrometry as a technology platform for studying conformational dynamics in large protein complexes

    Creator:
    Nirudodhi, Sasidhar N.
    Abstract:
    Proteins are essential to all biological systems. Proteins participate in numerous cellular processes by interacting with other proteins, other metabolites and membranes in a dynamic environment. Studying the structural and conformational properties of proteins in the solution phase is necessary to understand their protein folding and interaction dynamics. This research...
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    technically more advanced HDX MS experimental setup was used to study the exchange in properties of two