The general theme of this dissertation is the application of novel carbon based micro sensors with the scanning electrochemical microscopy (SECM). SECM allows positioning of the probe without touching the substrate while mapping the chemical parameters in 3D space above the substrate. The main focus of the dissertation is the...
Sporosarcina pasteurii is known to produce calcite or biocement in the presence of urea and Ca²⁺. Herein, we report the use of novel ultramicrosensors such as pH, Ca²⁺, and redox sensors, along with a scanning electrochemical microscope (SECM), to monitor a real-time, bacteria-mediated urea hydrolysis process and subsequent changes in...
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calcification process by
Sporosarcina pasteurii biofilm†
Dustin Harris,‡a Jyothir Ganesh Ummadi,‡a Andrew R
The general theme of this dissertation is the development of new analytical sensors to quantitatively study biological and environmental samples in aqueous and non-aqueous environments. A platinum ultramicroelectrode (UME) and a unique carbon-based pH microprobe were used to design a new SECM-based electrochemical bioassay. The Pt UME was used to...