Pre-existing Expressed Sequence Tag (EST) libraries (1,305 sequences) and a newly constructed microsatellite-enriched genomic library (136 clones) from the V. corymbosum cultivar 'Bluecrop' were screened for unique Simple Sequence Repeat (SSR)-containing sequences. Our goal was to develop a set of robust SSR markers for use in managing the National Clonal...
Improved recovery of cryopreserved meristems and calli from in
vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants
was obtained by two-hour pretreatment with sucrose, proline, abscisic
acid-responsive protein (RABP), and bovine serum albumin (BSA)
solutions. Meristems and calli cold-acclimatized for one week were
immersed in pretreatment...
This publication reports the results of a preliminary survey listing 527 forest-tree plantations in the western United States and Canada. Of these, 250 involve families of known parentage, 131 provenance-test
material, 87 clonal material, and 59 interspecific hybrids. The report is intended (1) to serve forest researchers in locating genetic...
In-vitro plants in slow-growth storage must be routinely evaluated for repropagation
and assessment of viability. It is often difficult to determine plant health by visual
assessment due to variation in growth patterns and plant structure among genera. In
order to evaluate cold-stored germplasm, laboratories need more effective approaches for
viability...
The accessions used to create the Snap Bean Diversity Panel (SnAP) were 378 snap bean cultivars and germplasm lines selected from North American and European germplasm. This panel supplements the 150 member Bean CAP snap bean diversity panel (SBDP) with an additional 228 snap bean accessions. The Bean CAP SBDP...
The accessions used to create the Snap Bean Diversity Panel were 150 snap bean (Phaseolus vulgaris) cultivars and breeding lines selected from North American and European germplasm to create a population that is representative of the snap bean market class from a historical and geographical viewpoint. This panel was developed...
Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic acid (LA), glycine betaine (GB) and polyvinylpyrrolidone (PVP) were applied at four steps of the PVS2 vitrification protocol (pretreatment, loading, rinsing, and regrowth). Shoot tips...
Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen. In the first study, 12 robust Simple Sequence Repeat (SSR) markers were developed from two microsatellite-enriched libraries of...
Barley (Hordeum vulgare L.) is one of the oldest known domesticated crops. Originally cultivated for human consumption, other end-uses have gained importance over the millennia. Barley is the fourth most important cereal crop in the world (FAO-STAT, 2011), and today it is mainly used as animal feed or malted for...