Pre-existing Expressed Sequence Tag (EST) libraries (1,305 sequences) and a newly constructed microsatellite-enriched genomic library (136 clones) from the V. corymbosum cultivar 'Bluecrop' were screened for unique Simple Sequence Repeat (SSR)-containing sequences. Our goal was to develop a set of robust SSR markers for use in managing the National Clonal...
A pathogenicity locus of Pseudomonas syringae pv.
syringae identified by Tn5 mutagenesis was investigated.
The mutant strain PS9024 is attenuated for disease
expression in its host, Phaseolus vulgaris, but produces
the hypersensitive reaction (HR) in the nonhost, tobacco
(Nicotina tabacum). A cosmid clone carrying 16 kilobases
(kb) of contiguous genomic...
The Mx protein family of rainbow trout (Oncorhynchus mykiss) was characterized at the molecular level. With primers derived from a partial sequence of Perch (Perca fluviatis) Mx genomic DNA, a partial rainbow trout Mx genomic DNA sequence was obtained by polymerase chain reaction (PCR) amplification. All salmonid fish investigated contained...
The auxin-resistant diageotropica (dgt) mutant of tomato (Lycopersicon esculentum Mill.) has a pleiotropic phenotype including a lack of lateral roots and reduced gravitropic response, apical dominance, vascular development, and fruit growth. The dgt mutation reduces the auxin sensitivity of only a subset of auxin responses while levels, metabolism, and transport...
Mutation of repetitive DNA by repeat-induced point mutation (RIP) is a process that occurs in many filamentous fungi of the Ascomycota during the sexual cycle. Concurrently, direct DNA repeats are often deleted by homologous recombination at high frequency during the sexual cycle. Thus, the processes of RIP and deletion compete...
Rhizopogon is a hypogeous fungal genus that forms ectomycorrhizae
with genera of the Pinaceae. The greatest number and species of
Rhizopogon are found in coniferous forests of the Pacific Northwestern
United States, where members of the Pinaceae are also concentrated.
Rhizopogon spp. are host-specific primarily with Pinus spp. and
Pseudotsuga...
A partial cystatin cDNA from rainbow trout was
generated by reverse transcription polymerase chain reaction
with two degenerate primers. The partial cystatin PCR
product was 168 bp and used to screen trout liver λgt 11
cDNA library. Four positive clones were isolated and
designated as cstl, cst2, cst3 and cst4....
Proteinase P-II purified from parasitized Pacific whiting muscle was previously
identified to be one form of cathepsin L. It appeared to be present in three isozymatic
forms on non-denaturing PAGE gel stained for activity. Its autolytic degradation was
observed on SDS-PAGE gel under its optimum condition, 55°C and pH 5.5,...