Index Catalog // ScholarsArchive@OSU

Creator:: Black, Margaret E.
Abstract:: Thymidine kinase is a key enzyme in the nucleotide salvage pathway, catalyzing the production of dTMP from thymidine and ATP. In order to identify the structural features of the TK protein and/or primary amino acid sequences which contribute to the catalytic and regulatory activities of this enzyme, an in vitro...
Resource Type:: Dissertation
Full Text:: Vaccinia Virus Thymidine Kinase Enzyme Abstract approved: Redacted for Privacy Dr. Dennis E. Hruby

Creator:: Shen, Rongkun
Abstract:: The enzymes of dNTP synthesis in T4 infection associate to form a multienzyme complex, the T4 dNTP synthetase complex, facilitating the flow of metabolites en route to dNTPs, and their subsequent flow into DNA. Study of protein-protein interactions helps one to understand how the enzymes in the complex are organized...
Resource Type:: Dissertation
Full Text:: intermediate from the catalytic site of one enzyme to the next, in preference to its release to the

Creator:: Bao, Kogan K.
Abstract:: Integrase catalyzes insertion of a retroviral genome into the host chromosome. Following reverse transcription, integrase binds specifically to the ends of the duplex retroviral DNA, endonucleolytically cleaves two nucleotides from each 3'-end (the processing activity), and inserts these ends into the host DNA (the joining activity) in a concerted manner....
Resource Type:: Dissertation
Full Text:: : Kinetic Analysis of Avian Sarcoma Virus Integrase in the Presteady-State. Abstract approved: Isaac Wong

Creator:: Kim, JuHyun
Abstract:: With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
Resource Type:: Dissertation
Full Text:: multiple enzyme complex and dNTP compartmentation in T4-infected E.coli

Creator:: Chaloupka, Kim A.
Abstract:: A kinetics-based enzyme-linked immunosorbent assay (KELA) for detection of serum IgG antibody against bovine respiratory syncytial virus (BRSV) was developed and optimized. Optimization factors tested included brand of microELISA plate, length of incubation periods at various temperatures, type of conjugate, purified versus precipitated antigen preparations, type of dilution buffer as...
Resource Type:: Masters Thesis
Full Text:: incubation and rinsing, substrate for the enzyme is added. If the enzyme is bound (that is, virus-specific

Creator:: Whitworth, Jonathan L.
Abstract:: Potato leafroll virus (PLRV) causes yield and quality losses in potato. PLRV is identified by plant symptoms and serological tests such as an enzyme-linked immunosorbent assay (ELISA). A similar serological test, direct tissue blotting assay (DTBA), was used to detect and monitor PLRV movement in field-inoculated Russet Burbank plants and...
Resource Type:: Dissertation
Full Text:: roll virus (PLRV) tests from visual inspection, direct-tissue blotting and enzyme-linked immunosorbent

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