The use of radioactive isotopes as tracers in biological systems
has become widespread since the close of World War II. Proper
use of radiotracers requires a fundamental understanding of the physical
nature of radioactivity, the characteristics of ionizing radiation,
and the various methods available for measuring radioactivity.
More importantly, the...
The effects of different nitrogen sources on the primary and secondary pathways of Azotobacter vinelandii cells were studied by means of the radiorespirometric method. C¹⁴ labeled glucose and several key amino acids related to the tricarboxylic acid cycle, such as glutamic acid, aspartic acid, alanine, serine and glycine were used...
An attempt has been made to elucidate the biosynthetic precursor
of ethylene in the mold P. digitatum. By application of basic
radiotracer methodology and the utilization of several specifically
labeled radiochemical substrates, it has been possible to demonstrate
that the internal carbon atoms of C-4 dicarboxylic acids such
as succinate,...