Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an
enzyme unique to the deoxyribonucleotide metabolism of T-even
bacteriophages. To study biochemical and biophysical properties of
the enzyme, as well as the interaction of dCMP hydroxymethylase with
other DNA precursor biosynthetic enzymes in vitro, availability of large
amounts of the enzyme is...
Due to the advantages of the high surface area and high porosity, Metal-Organic Framework (MOF) materials are widely used in gas storage. As a typical MOF material, Zeolitic Imidazolate Framework-8 (Zif-8) has high selectivity and adsorption capability of carbon dioxide (CO2). In this thesis, we investigated the infrared absorption of...
Dysfunction of mitochondria has been linked to aging and the pathogenesis of many degenerative diseases. Aside from their primary function in energy production, mitochondria are considered as a major source and target of reactive oxygen and nitrogen species (ROS/RNS) in cells as well. The mitochondrial thiol proteome is a subset...
Rising energy demands and the continual push to find more energy efficient technologies have been the impetus for the investigation of waste heat recovery techniques. Diesel engine exhaust heat utilization has the potential to significantly reduce the consumption of fossil fuels and reduce the release of greenhouse gases, because diesel...
The role of volumetric energy density on the microstructural evolution, texture and mechanical properties of 304L stainless steel parts additively manufactured via selective laser melting process is investigated. 304L is chosen because it is a potential candidate to be used as a matrix in a metal matrix composite with nanoparticles...
A nickel-based Hastelloy C22 powder with a nominal composition of Ni-21.30Cr-13.36Mo-1.92Co-2.94W-3.85Fe-0.23Mn-0.3V(wt%) and particle size distribution of D₁₀=23.8±0.3 μm, D₅₀=37.5±0.2μm, and D₉₀=59.3±1.1 μm was gas atomized to be used as a feedstock in Laser Powder Bed Fusion (LPBF). In this study, laser power and scanning speed were optimized to manufacture specimens...
The ultimate goal of this work is to analyze and compare two predictive models of thermal profile in laser processing of materials. An analytical method that uses closed-form equations to represent a geometric model of a laser heat source will be contrasted with a commercial computational fluid dynamics code that...
We describe a series of novel computational models, CERENKOV (Computational Elucidation of the REgulatory NonKOding Variome) and its successors CERENKOV2, CERENKOV3, and Convolutional CERENKOV3, for discriminating regulatory single nucleotide polymorphisms (rSNPs) from non-regulatory SNPs within non-coding genetic loci. The CERENKOV models are designed for recognizing rSNPs in the context of...
Chain saws are a common tool used by large logging corporations and family homesteads to manage forests, limb trees and buck lumber. A significant cost of operation is the continuous required supply of bar and chain oil. Consumers and logging corporations desire a lubricant with a high performance to cost...