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Filtering by: Funding statement We would like to acknowledge the support of National Institutes of Health Grant R01 GM050389 to L.B.P. and P.A.K. Remove constraint Funding statement: We would like to acknowledge the support of National Institutes of Health Grant R01 GM050389 to L.B.P. and P.A.K.

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  • Functional, structural and evolutionary analyses of the ferritin-like superfamily of proteins

    Creator:
    Cooley, Richard Bleier
    Abstract:
    The ferritin-like superfamily (FLSF) of proteins is composed of a wide variety of functionally diverse proteins involved in oxygen dependent metal-mediated electron transfer reactions. Their biological importance is exemplified by the fact FLSF proteins are found in almost every organism from all three domains of life. Their functions range from...
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    Dissertation
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    home-cooked food. I would like to acknowledge the National Institutes of Health grant numbers

  • Biophysical analyses of peroxiredoxins and a partner reductase

    Creator:
    Hall, Andrea Renée
    Abstract:
    Peroxiredoxins (Prxs) are dominant peroxide-reducing enzymes with two important roles: they protect all organisms from oxidative damage induced by peroxides, and in eukaryotes, they participate in hydrogen peroxide signaling pathways. This dissertation presents studies aimed at the biophysical characterization of select Prxs and a Prx reductase to elucidate their structure-function...
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    Dissertation
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    you. I would like to acknowledge the National Institutes of Health for providing the funding that

  • Peroxiredoxins in Parasites

    Creator:
    Gretes, Michael C., Poole, Leslie B., and Karplus, P. Andrew
    Abstract:
    Significance: Parasite survival and virulence relies on effective defenses against reactive oxygen and nitrogen species produced by the host immune system. Peroxiredoxins (Prxs) are ubiquitous enzymes now thought to be central to such defenses and, as such, have potential value as drug targets and vaccine antigens. Recent Advances: Plasmodial and...
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    Article
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    humans and are major contributors to the global burden of disease (267). In these organisms, Prxs are

  • Peroxiredoxins: Guardians Against Oxidative Stress and Modulators of Peroxide Signaling

    Creator:
    Perkins, Arden, Nelson, Kimberly J., Parsonage, Derek, Poole, Leslie B., and Karplus, P. Andrew
    Abstract:
    Peroxiredoxins (Prxs) are a ubiquitous family of cysteine-dependent peroxidase enzymes that play dominant roles in regulating peroxide levels within cells. These enzymes, often present at high levels and capable of rapidly clearing peroxides, display a remarkable array of variations in their oligomeric states and susceptibility to regulation by hyperoxidative inactivation...
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    important contributions due to space limitations. Support from the National Institutes of Health (R01

  • Tuning of Peroxiredoxin Catalysis for Various Physiological Roles

    Creator:
    Perkins, Arden, Poole, Leslie B., and Karplus, P. Andrew
    Abstract:
    Peroxiredoxins (Prxs) make up an ancient family of enzymes that are the predominant peroxidases for nearly all organisms and play essential roles in reducing hydrogen peroxide, organic hydroperoxides, and peroxynitrite. Even between distantly related organisms, the core protein fold and key catalytic residues related to its cysteine-based catalytic mechanism have...
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    Article
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    of Health grant RO1 12 GM050389 to L.B.P. and P.A.K. 13 14 SUPPORTING INFORMATION 15 Table

  • Model systems for structural investigations into peroxiredoxin catalysis, conformation change, and inactivation

    Creator:
    Perkins, Arden
    Abstract:
    Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide, peroxynitrous acid, and organic peroxides, and are extremely efficient peroxidases, with k[subscript cat]/K[subscript M] on the order of 10⁷ - 10⁸ M⁻¹ s⁻¹. Besides their role in oxidative stress defense, evidence has accumulated that some eukaryotes, including humans, use Prxs as...
    Resource Type:
    Dissertation
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    , and dead garter snakes than working on the family farm. I would like to thank my Grandma Gloria for

  • Hydrogen/deuterium exchange mass spectrometry as a technology platform for studying conformational dynamics in large protein complexes

    Creator:
    Nirudodhi, Sasidhar N.
    Abstract:
    Proteins are essential to all biological systems. Proteins participate in numerous cellular processes by interacting with other proteins, other metabolites and membranes in a dynamic environment. Studying the structural and conformational properties of proteins in the solution phase is necessary to understand their protein folding and interaction dynamics. This research...
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    Dissertation
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    people in the mass spectrometry lab for their support and assistance. I would like to acknowledge the