Partial purification of diamine oxidase from pea seedlings,
Pisum sativum, was accomplished by homogenization of 8-10 day
etiolated epicotyl tissue, followed by ammonium sulfate fractionation
and DEAE Sephadex column chromatography. The preparation thus
obtained was purified 50 fold.
Some properties of this enzyme were investigated. A radio-tracer method was adapted...
Some kinetics of the oxygen requirement of amine oxidases
have been investigated using bovine plasma monoamine oxidase (PAO)
and pea seedling diamine oxidase (DAO). A system is described for
studying enzyme reactions under controlled oxygen tension and in an
O₂-free N₂ atmosphere.
O₂ Km values were determined at two different...
Several aspects of the potent inhibition of bovine plasma amine
oxidase (PAO) by hydrazines were investigated by kinetic and preparative
means. The inhibition was classified as pseudo-irreversible
by the method of Ackerman and Potter (Proc. Soc. Exptl. Biol. Med.
72, 1 (1949), and was found to exhibit Zone B kinetic...
The purpose of this research was to compare four different media that were used to monitor SOC atmospheric concentrations in remote ecosystems. The accumulation of semi-volatile organic compounds, including pesticides, polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), was investigated in lichen, 2-year old conifer needles, resin-based passive air sampling...