Dietary iodine may play an important role in the nutritional health of freshwater fish larvae. Artemia, commonly used for the culture of larval zebrafish (Danio rerio), contain low concentrations of iodine when compared with wild zooplankton. Water-soluble micronutrients, such as iodine, are difficult to deliver to Artemia due to rapid...
Vitamin E was first described in 1922 as an unknown factor required for impregnated rats to carry their offspring to term. In fact, when vitamin E was chemically characterized it was given the name "tocopherol" derived from the Greek: tokos = childbirth; phero = to bear; and –ol, indicating an...
The use of zebrafish in behavioral neuroscience is rapidly growing. Zebrafish can be assessed for alterations in multiple behavioral endpoints, creating opportunities to use this powerful model to identify chemicals that alter behavioral phenotypes. To evaluate the utility of zebrafish for neurotoxicity research, we designed custom instrumentation to evaluate numerous...
Graphene and its derivatives are two-dimensional hexagonal lattices that possess desirable physicochemical properties leading to an ever-expanding list of industrial and biomedical uses. Graphene oxides (GOs) are becoming a popular graphene alternative and while there have been human safety studies, investigations of developmental toxicity are lacking. The use of distinct...
As nanoparticle (NP) research and development expands, the significance of NP biodistribution effects on toxicity increases. A reliable method for measuring NP biodistribution is critical. Hyperspectral Imaging (HSI) is capable of accurately quantitating NP biodistribution within embryonic zebrafish with incredibly high precision. Before HSI processing, the embryo must be sectioned...
A decline in the oxygen cost of exercise enhances exercise tolerance and performance. Substantial research has shown that dietary nitrate lowers the oxygen cost of exercise in sedentary humans; however, the metabolic determinants regarding how dietary nitrate influences oxygen consumption in skeletal muscle is not known. We addressed this gap...
Since its inception as a laboratory animal in the early 1970s, the zebrafish has proven itself a rising star in the world of comparative biomedical sciences due to its short generation time, ease of care, external fertilization, and transparent larvae. In a very few decades, the zebrafish has been utilized...
Zebrafish (Brachydanio rerio) are small, freshwater teleost fishes in the family
Cyprinidae, the true minnows. They are native to the tropical latitudes of India, but
have become widespread through their use as aquarium fish and as models for several
branches of biological research. Their ease of rearing, short generation time,...
Zebrafish, Danio rerio, are widely used as vertebrate research organisms yet little is know about their responses to husbandry stressors. This dissertation examined the whole-body cortisol responses of zebrafish to husbandry stress. Additionally, the effects of stress on the susceptibility to mycobacteriosis and microsporidiosis (Pseudoloma neurophilia) were determined including effects...
Utilization of zebrafish, Danio rerio, has steadily increased and its applications have expanded into numerous fields of science. Applying elevated temperatures (32°C to 37°C) to this organism has allowed researchers to conduct climate change, human cancer and infectious disease studies. Though zebrafish can be acclimated from the standard 28°C to...