A label-free, photoluminescence (PL) based biosensor for 2,4,6-trinitrotoluene (TNT) detection was developed by functionalizing diatom biosiilica with a TNT-specific single-chain variable fragment (scFv). The scFv loading was estimated to be 0.040±0.003 (μg scFv/μg biosilica). In saturated concentration, TNT binding to scFvbiosilica quenched about 13% of its PL. Dose response follows...
The phosphoprotein (P protein) in viruses of the Mononegavirales order is known to play an essential role in the completion of viral replication mechanisms. The P protein is involved in stabilizing the viral polymerase and nucleoprotein during RNA transcription and replication, regulating nascent RNA encapsidation by the nucleoprotein, and mediation...
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Zhen Yu, Committee Member, representing Department of Biochemistry and Biophysics
Hydrogen cyanamide (CY) induced earlier budbreak, more
uniform and a higher percentage of budbreak in resting
poplar (Populus alba x P. gradidentata, NC 5339) plants.
However, it was phytotoxic to poplar when applied at 0.5 M
or higher concentrations. Percent budbreak increased and
percent dieback decreased when poplar plants were...
A selective and label-free biosensor for detection of the explosive compound 2,4,6-trinitrotoluene (TNT) in aqueous solution was developed based on the principle of photoluminescence quenching of upon immunocomplex formation with antibody-functionalized diatom frustule biosilica. The diatom frustule is an intricately nanostructured, highly porous biogenic silica material derived from the shells...
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biosilica functionalized with an anti-TNT monoclonal
antibody fragment
LeZhen a, Nicole Ford b, Debra K
A selective and label-free biosensor for detection of the explosive compound 2,4,6-trinitrotoluene (TNT) in aqueous solution was developed based on the principle of photoluminescence quenching of upon immunocomplex formation with antibody-functionalized diatom frustule biosilica. The diatom frustule is an intricately nanostructured, highly porous biogenic silica material derived from the shells...
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an anti-TNT monoclonal antibody fragment
Le Zhena, Nicole Fordb, Debra K. Galea, Guritno
A selective and label-free biosensor for detection of the explosive compound 2,4,6-trinitrotoluene (TNT) in aqueous solution was developed based on the principle of photoluminescence quenching of upon immunocomplex formation with antibody-functionalized diatom frustule biosilica. The diatom frustule is an intricately nanostructured, highly porous biogenic silica material derived from the shells...
Culture, thought worldview and language have been
discussed for a long time in different fields from various
perspectives. However, the basis of this study is the view
of language as both the product and producer of people just
as people are the producer and product of language. Each
language requires...
It is well known that in mice the extension in lifespan by rapamycin is sexually dimorphic, in that it has a larger effect in females than males. In a previous study we showed that in male C57BL6 mice, rapamycin had less profound effects in both gene expression and liver metabolites...
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observed under dietary restriction
Zhen Yu1, Bharath Sunchu2, Wilson C Fok3, Nahla Alshaikh2 and Viviana I
Coring/logging data and physical property measurements from International Ocean Discovery Program Expedition 349 are integrated with, and correlated to, reflection seismic data to map seismic sequence boundaries and facies of the central basin and neighboring regions of the South China Sea. First-order sequence boundaries are interpreted, which are Oligocene/Miocene, middle...
Cancer is class of diseases that has great diversity and unfortunately is one of the leading causes of death in the United States. Chemotherapy remains an important cancer treatment modality, and superior chemotherapeutic drugs are constantly being sought. Gemcitabine is an anti-tumor chemotherapeutic drug and it is the first line...
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, Nijaporn Yanasarn, Zhen Yu, Letty
Rodriguez, Xinran Li, Ziyi Ji. A special thanks to my advisor, Wanda