Using comparative genomics to develop a molecular diagnostic for the identification of an emerging pest Drosophila suzukii Public Deposited

http://ir.library.oregonstate.edu/concern/articles/00000163g

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by Cambridge University Press and can be found at:  http://journals.cambridge.org/action/displayJournal?jid=BER

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Drosophila suzukii (Spotted Wing Drosophila) has recently become a serious invasive pest of fruit crops in the USA, Canada, and Europe, leading to substantial economic losses. D. suzukii is a direct pest, ovipositing directly into ripe or ripening fruits; in contrast, other Drosophilids utilize decaying or blemished fruits and are nuisance pests at worst. Immature stages of D. suzukii are difficult to differentiate from other Drosophilids, posing problems for research and for meeting quarantine restrictions designed to prevent the spread of this pest in fruit exports. Here we used a combined phylogenetic and bioinformatic approach to discover genetic markers suitable for a species diagnostic protocol of this agricultural pest. We describe a molecular diagnostic for rapid identification of single D. suzukii larva using multiplex polymerase chain reaction. Our molecular diagnostic was validated using nine different species of Drosophila for specificity and 19 populations of D. suzukii from different geographical regions to ensure utility within species.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Murphy, K. A., Unruh, T. R., Zhou, L. M., Zalom, F. G., Shearer, P. W., Beers, E. H., ... & Chiu, J. C. (2015). Using comparative genomics to develop a molecular diagnostic for the identification of an emerging pest Drosophila suzukii. Bulletin of entomological research, 105(3), 364-372. doi:10.1017/S0007485315000218
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2015-06-24T16:33:38Z (GMT) No. of bitstreams: 3 WaltonV.HorticultureUsingComparativeGenomics .pdf: 726264 bytes, checksum: be6e343864c4b2a6002fc3feb95d963b (MD5) WaltonV.HorticultureUsingComparativeGenomicsSupplementaryMaterialTableS1.xlsx: 281489 bytes, checksum: a84d8650a3ce98c0d8c37bc785e41a04 (MD5) WaltonV.HorticultureUsingComparativeGenomicsSupplementaryMaterialTableS2.xlsx: 342050 bytes, checksum: 450fbe9b611169dd39e4cbf5294da069 (MD5)
  • description.provenance : Made available in DSpace on 2015-06-24T16:33:38Z (GMT). No. of bitstreams: 3 WaltonV.HorticultureUsingComparativeGenomics .pdf: 726264 bytes, checksum: be6e343864c4b2a6002fc3feb95d963b (MD5) WaltonV.HorticultureUsingComparativeGenomicsSupplementaryMaterialTableS1.xlsx: 281489 bytes, checksum: a84d8650a3ce98c0d8c37bc785e41a04 (MD5) WaltonV.HorticultureUsingComparativeGenomicsSupplementaryMaterialTableS2.xlsx: 342050 bytes, checksum: 450fbe9b611169dd39e4cbf5294da069 (MD5) Previous issue date: 2015-06
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2015-06-24T16:33:21Z No. of bitstreams: 3 WaltonV.HorticultureUsingComparativeGenomics .pdf: 726264 bytes, checksum: be6e343864c4b2a6002fc3feb95d963b (MD5) WaltonV.HorticultureUsingComparativeGenomicsSupplementaryMaterialTableS1.xlsx: 281489 bytes, checksum: a84d8650a3ce98c0d8c37bc785e41a04 (MD5) WaltonV.HorticultureUsingComparativeGenomicsSupplementaryMaterialTableS2.xlsx: 342050 bytes, checksum: 450fbe9b611169dd39e4cbf5294da069 (MD5)

Relationships

In Administrative Set:
Last modified: 07/26/2017 Default
Citations:

EndNote | Zotero | Mendeley