Article

 

Paired-End Analysis of Transcription Start Sites in Arabidopsis Reveals Plant-Specific Promoter Signatures Public Deposited

https://ir.library.oregonstate.edu/concern/articles/02870x71g

This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Society of Plant Biologists and can be found at:  http://www.plantcell.org/

Descriptions

Attribute NameValues
Creator
Abstract
  • Understanding plant gene promoter architecture has long been a challenge due to the lack of relevant large-scale data sets and analysis methods. Here we present a publicly available, large-scale transcription start site (TSS) dataset in plants using a high-resolution method for analysis of 5’ ends of mRNA transcripts. Our dataset is produced using the Paired-End Analysis of Transcription Start Sites (PEAT) protocol, providing millions of TSS locations from wild-type Col-0 Arabidopsis whole root samples. Using this dataset, we grouped TSS reads into “TSS tag clusters” and categorized clusters into three spatial initiation patterns: narrow peak, broad with peak, and weak peak. We then designed a machine learning model that predicts the presence of TSS tag clusters with outstanding sensitivity and specificity for all three initiation patterns. We used this model to analyze the transcription factor binding site content of promoters exhibiting these initiation patterns. In contrast to the canonical notions of TATA-containing and more broad “TATA-less” promoters, the model shows that, in plants, the vast majority of transcription start sites are TATA-free, and are defined by a large compendium of known DNA sequence binding elements. We present results on the usage of these elements, and provide our Plant PEAT Peaks (3PEAT) model that predicts the presence of TSSs directly from sequence.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Morton, T., Petricka, J., Corcoran, D. L., Li, S., Winter, C. M., Carda, A., ... & Megraw, M. (2014). Paired-end analysis of transcription start sites in Arabidopsis reveals plant-specific promoter signatures. The Plant Cell, 26(7), 2746-2760. doi:10.1105/tpc.114.125617
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
  • M.M. was supported by an NIH K99-R00 Pathway to Independence Award GM097188. T.M. was supported by startup funds from Oregon State University. J.J.P. is supported by a NIH Ruth L. Kirschstein NRSA award (F32GM086976). P.N.B. and U.O. are funded by NSF award IOS- 1021619. U.O additionally acknowledges support from NSF award MCB-0822033.
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2015-01-29T01:35:55Z (GMT). No. of bitstreams: 1 MegrawMollyBotanyPlantPathologyPairedEndAnalysis.pdf: 813175 bytes, checksum: aeeefe1f24ebc7a249bed28960600089 (MD5) Previous issue date: 2014-07
  • description.provenance : Submitted by Deanne Bruner (deanne.bruner@oregonstate.edu) on 2015-01-29T01:35:18Z No. of bitstreams: 1 MegrawMollyBotanyPlantPathologyPairedEndAnalysis.pdf: 813175 bytes, checksum: aeeefe1f24ebc7a249bed28960600089 (MD5)
  • description.provenance : Approved for entry into archive by Deanne Bruner(deanne.bruner@oregonstate.edu) on 2015-01-29T01:35:55Z (GMT) No. of bitstreams: 1 MegrawMollyBotanyPlantPathologyPairedEndAnalysis.pdf: 813175 bytes, checksum: aeeefe1f24ebc7a249bed28960600089 (MD5)

Relationships

Parents:

This work has no parents.

Items