Article
 

Ammonium uptake and incorporation by Chesapeake Bay phytoplankton: Short term uptake kinetics

Public Deposited

Contenu téléchargeable

Télécharger le fichier PDF
https://ir.library.oregonstate.edu/concern/articles/0c483k99z

Descriptions

Attribute NameValues
Creator
Abstract
  • Using 15N-labeled NH4+, we assessed the time-course of NH4+ uptake, the parameters of saturation kinetics, and the rates of incorporation of NH4+ into trichloroacetic acid-insoluble material by Chesapeake Bay phytoplankton. The amount of NH4+ taken up by the phytoplankton increased linearly with time for 2 h for all samples growing on or exposed to high concentrations (> 1 μg-atom N • liter-1) of NH4+, but extrapolations to time zero did not intercept the origin. These results could be explained by rapid isotopic equilibration of internal and external NH4+ pools. For stations for which ambient NH4+ bordered on detection limits, rapid but nonsaturablc uptake was observed in l- and 5-min incubations, whereas saturation was attained in 15–60-min incubations. The experiments on rates of NH4+ incorporation into TCA-insoluble material demonstrated that most (>85%) of the 15N label was incorporated into the macromolecular fraction by ≈15 min. These data thus demonstrate that rates of NH4+ uptake may become limited within minutes by rates of assimilation and incorporation into macromolecules and that reported kinetic parameters reflect these metabolic rates rather than transport rates.
Resource Type
DOI
Date Issued
Citation
  • Wheeler, Patricia A., Glibert, Patricia M., McCarthy, James J., ( 1982), Ammonium uptake and incorporation by Chesapeake Bay phytoplankton: Short term uptake kinetics, Limnology and Oceanography, 27, doi: 10.4319/lo.1982.27.6.1113.
Journal Title
Journal Volume
  • 27
Journal Issue/Number
  • 6
Déclaration de droits
Funding Statement (additional comments about funding)
  • This work was supported by grant OCE 78-26011 and OCE 80-22990 from the National Science Foundation.
Publisher
Peer Reviewed
Language
Replaces
ISSN
  • ISSN: 0024-3590

Des relations

Parents:

This work has no parents.

Articles

La vignette Titre Date de téléchargement Visibilité actes
file details Wheeler_et._al._1982a.pdf 2017-07-24 Public Télécharger