Endogenous Small RNA Mediates Meiotic Silencing of a Novel DNA Transposon Public Deposited

http://ir.library.oregonstate.edu/concern/articles/0k225c96m

Supporting information available online at:  http://g3journal.org/content/5/10/1949/suppl/DC1

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  • Genome defense likely evolved to curtail the spread of transposable elements and invading viruses. A combination of effective defense mechanisms has been shown to limit colonization of the Neurospora crassa genome by transposable elements. A novel DNA transposon named Sly1-1 was discovered in the genome of the most widely used laboratory “wild-type” strain FGSC 2489 (OR74A). Meiotic silencing by unpaired DNA, also simply called meiotic silencing, prevents the expression of regions of the genome that are unpaired during karyogamy. This mechanism is posttranscriptional and is proposed to involve the production of small RNA, so-called masiRNAs, by proteins homologous to those involved in RNA interference - silencing pathways in animals, fungi, and plants. Here, we demonstrate production of small RNAs when Sly1-1 was unpaired in a cross between two wild-type strains. These small RNAs are dependent on SAD-1, an RNA-dependent RNA polymerase necessary for meiotic silencing. We present the first case of endogenously produced masiRNA from a novel N. crassa DNA transposable element.
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  • Wang, Y., Smith, K. M., Taylor, J. W., Freitag, M., & Stajich, J. E. (2015). Endogenous small RNA mediates meiotic silencing of a novel DNA transposon. G3: Genes| Genomes| Genetics, 5(10), 1949-1960. doi:10.1534/g3.115.017921
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  • description.provenance : Made available in DSpace on 2015-11-12T15:17:41Z (GMT). No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) SmithKristinaBiochemBiophysicsEndogenousSmallRNA.pdf: 2879789 bytes, checksum: 3ba003fd5214a4b336ea4a2daebcd734 (MD5) Previous issue date: 2015-10-01
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2015-11-12T15:17:41Z (GMT) No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) SmithKristinaBiochemBiophysicsEndogenousSmallRNA.pdf: 2879789 bytes, checksum: 3ba003fd5214a4b336ea4a2daebcd734 (MD5)

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