| Creator |
|
|---|
| Abstract or Summary |
- Zebrafish are a powerful model organism to study disease. Like other animal models, Danio rerio colonies are
at risk of pathogenic infection. Microsporidia, a group of intracellular fungus-like parasites, are one potential
threat. Microsporidian spores germinate and spread causing pathological changes in the central nervous system,
skeletal muscle, and other anatomic sites. Infection can impair breeding, cause other morbidities, and ultimately
be lethal. Previously, detecting microsporidia in zebrafish has required sacrificing animals for histopathologic
analysis or microscopic examination of fresh tissues. Here, we show that fish with microsporidial infection often
have autofluorescent nodules, and we demonstrate infectious spread from nodule-bearing fish to healthy
D. rerio. Histologic analyses revealed that fluorescent nodules are granulomatous lesions composed of spores,
degenerating muscle, and inflammatory cells. Additional histologic staining verified that microsporidia were
present, specifically, Pseudoloma neurophilia. Polymerase chain reaction (PCR)-based testing confirmed the
presence of P. neurophilia. Further PCR testing excluded infection by another common zebrafish microsporidial
parasite, Pleistophora hyphessobryconis. Collectively, these studies show that P. neurophilia can induce
skeletal muscle granulomas in D. rerio, a previously unknown finding. Moreover, since granulomas autofluoresce,
microscopic screening for P. neurophilia infection is feasible in live fish, avoiding the need to
sacrifice fish for surveillance for this pathogen.
|
|---|
| Resource Type |
|
|---|
| DOI |
|
|---|
| Date Available |
|
|---|
| Date Issued |
|
|---|
| Citation |
- West, K., Miles, R., Kent, M. L., & Frazer, J. K. (2014). Unusual Fluorescent Granulomas and Myonecrosis in Danio Rerio Infected by the Microsporidian Pathogen Pseudoloma Neurophilia. Zebrafish, 11(3), 283-290. doi:10.1089/zeb.2013.0933
|
|---|
| Series |
|
|---|
| Rights Statement |
|
|---|
| Publisher |
|
|---|
| Peer Reviewed |
|
|---|
| Language |
|
|---|
| Replaces |
|
|---|
| Additional Information |
- description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-07-28T18:41:16Z (GMT) No. of bitstreams: 2
KentMichaelMicrobiologyUnusualFluorescentGranulomas.pdf: 932856 bytes, checksum: 09f173e8212ffae857758b7250bf7e98 (MD5)
KentMichaelMicrobiologyUnusualFluorescentGranulomas_FiguresS1-S3.zip: 740849 bytes, checksum: f4b18e5b5d70a05f9f7d10c75efbf1a6 (MD5)
- description.provenance : Made available in DSpace on 2014-07-28T18:41:16Z (GMT). No. of bitstreams: 2
KentMichaelMicrobiologyUnusualFluorescentGranulomas.pdf: 932856 bytes, checksum: 09f173e8212ffae857758b7250bf7e98 (MD5)
KentMichaelMicrobiologyUnusualFluorescentGranulomas_FiguresS1-S3.zip: 740849 bytes, checksum: f4b18e5b5d70a05f9f7d10c75efbf1a6 (MD5)
Previous issue date: 2014-06-11
- description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-07-28T18:40:47Z
No. of bitstreams: 2
KentMichaelMicrobiologyUnusualFluorescentGranulomas.pdf: 932856 bytes, checksum: 09f173e8212ffae857758b7250bf7e98 (MD5)
KentMichaelMicrobiologyUnusualFluorescentGranulomas_FiguresS1-S3.zip: 740849 bytes, checksum: f4b18e5b5d70a05f9f7d10c75efbf1a6 (MD5)
|
|---|
