- Structured Abstract -
To assess the effect of transfusion using a syringe and microaggregate filter on short-term
survival and circulating half-life of autologous feline RBCs.
Prospective, internally controlled, observational study.
A University Teaching Hospital
Six apparently healthy, owned pet cats.
Blood collection by jugular venipuncture. Transfusion with labeled, autologous, fresh red
MEASUREMENTS AND MAIN RESULTS:
Anticoagulated whole blood (35 ml/cat) was collected in two equal aliquots. RBCs were
washed and labeled at two different biotin densities, before suspension in autologous
plasma. Labeled RBCs were then transfused using two methods, gravity flow and pump
delivery using a 20ml syringe and 18 μm microaggregate filter.
Whole blood samples were collected from each cat at 2 hr intervals for 12 hours
following completion of the transfusions. Additional samples were collected at weekly
intervals up to 6 weeks to assess circulating half-life of the transfused cells. Cell survival
was assessed via flow cytometry. The proportion of transfused cells remaining in each
of the two populations was measured. Quantitative changes in the two populations over
time were assessed by two-way repeated measures ANOVA.
Biotinylated RBCs were readily detected in all cats over the 6-week sampling period.
There was a significant decrease in both populations of labeled cells over the 6-week
period (p<0.01), as expected. There was no difference in probability that the RBCs
would survive up to 12 hours immediately following transfusion, and no significant
difference in survival between the two groups over 6 weeks. The average half-life of all
labeled cells was approximately 23 days.
We conclude that, in contrast to findings from dogs, transfusion of autologous feline
RBCs using a syringe + aggregate filter method does not significantly impact short- or
long-term survival of the transfused cells.