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  • Structured Abstract - OBJECTIVE: To assess the effect of transfusion using a syringe and microaggregate filter on short-term survival and circulating half-life of autologous feline RBCs. DESIGN: Prospective, internally controlled, observational study. SETTING: A University Teaching Hospital ANIMALS: Six apparently healthy, owned pet cats. INTERVENTIONS: Blood collection by jugular venipuncture. Transfusion with labeled, autologous, fresh red blood cells. MEASUREMENTS AND MAIN RESULTS: Anticoagulated whole blood (35 ml/cat) was collected in two equal aliquots. RBCs were washed and labeled at two different biotin densities, before suspension in autologous plasma. Labeled RBCs were then transfused using two methods, gravity flow and pump delivery using a 20ml syringe and 18 μm microaggregate filter. Whole blood samples were collected from each cat at 2 hr intervals for 12 hours following completion of the transfusions. Additional samples were collected at weekly intervals up to 6 weeks to assess circulating half-life of the transfused cells. Cell survival was assessed via flow cytometry. The proportion of transfused cells remaining in each of the two populations was measured. Quantitative changes in the two populations over time were assessed by two-way repeated measures ANOVA. Biotinylated RBCs were readily detected in all cats over the 6-week sampling period. There was a significant decrease in both populations of labeled cells over the 6-week period (p<0.01), as expected. There was no difference in probability that the RBCs would survive up to 12 hours immediately following transfusion, and no significant difference in survival between the two groups over 6 weeks. The average half-life of all labeled cells was approximately 23 days. CONCLUSIONS: We conclude that, in contrast to findings from dogs, transfusion of autologous feline RBCs using a syringe + aggregate filter method does not significantly impact short- or long-term survival of the transfused cells.
  • Keywords: streptavidin-phycoerythrin, mechanical perfusion, cats, half-life, biotinylation, blood administration, flow cytometry
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