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Peroxisome proliferator-activated receptor / does not regulate glucose uptake and lactose synthesis in bovine mammary epithelial cells cultivated in vitro

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  • The hypothesis of the study was that inhibition of PPAR/ increases glucose uptake and lactose synthesis in bovine mammary epithelial cells by reducing the expression of the glucose transporter mRNA destabiliser calreticulin. Three experiments were conducted to test the hypothesis using immortalised bovine mammary alveolar (MACT) and primary bovine mammary (PBMC) cells. In Experiment 1, the most effective dose to inhibit PPAR/ activity among two synthetic antagonists (GSK-3787 and PT-s58) was assessed using a gene reporter assay. In Experiment 2, the effect on glucose uptake and lactose synthesis was evaluated by measuring glucose and lactose in the media and expression of related key genes upon modulation of PPAR/ using GSK-3787, the synthetic PPAR/ agonist GW-501516, or a combination of the two in cells cultivated in plastic. In Experiment 3, the same treatments were applied to cells cultivated in Matrigel and glucose and lactose in media were measured. In Experiment 1 it was determined that a significant inhibition of PPAR/ in the presence or absence of fetal bovine serum was achieved with 1000 nm GSK-3787 but no significant inhibition was observed with PT-s58. In Experiment 2, inhibition of PPAR/ had no effect on glucose uptake and lactose synthesis but they were both increased by GW-501516 in PBMC. The mRNA abundance of PPAR/ target gene pyruvate dehydrogenase kinase 4 was increased but transcription of calreticulin was decreased (only in MACT cells) by GW-501516. Treatment with GSK-3787 did not affect the transcription of measured genes. No effects on glucose uptake or lactose synthesis were detected by modulation of PPAR/ activity on cells cultivated in Matrigel. The above data do not provide support for the original hypothesis and suggest that PPAR/ does not play a major role in glucose uptake and lactose synthesis in bovine mammary epithelial cells.
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  • 85
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  • 0022-0299
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