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Measurement of Phospholipid Lateral Diffusion at High Pressure by In Situ Magic-angle Spinning NMR Spectroscopy

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https://ir.library.oregonstate.edu/concern/articles/2r36v7242

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  • The development of experimental methodologies that enable investigations of biochemistry at high pressure promises to yield significant advances in our understanding of life on Earth and its origins. Here, we introduce a method for studying lipid membranes at thermodynamic conditions relevant for life at deep sea hydrothermal vents. Using in situ high pressure magic-angle spinning solid state nuclear magnetic resonance spectroscopy (NMR), we measure changes in the fluidity of model microbial membranes at pressures up to 28 MPa. We find that the fluid-phase lateral diffusion of phospholipids at high pressure is significantly affected by the stoichiometric ratio of lipids in the membrane. Our results were facilitated by an accessible pressurization strategy that we have developed to enable routine preparation of solid state NMR rotors to pressures of 30 MPa or greater.
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  • 8
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  • 1
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  • This work was supported by an Arnold O. Beckman Postdoctoral Fellowship in Chemical Instrumentation, awarded to T.O.P, and by the US National Institutes of Health (R01-GM139905 to A.J.N.). We thank Peter Macdonald (University of Toronto) for helpful discussions about CODEX lateral diffusion data analysis. Additionally, we thank Kevin Geohring (Blue Sky NMR, LLC) for advice and support in troubleshooting the interface to the temperature controller used in our NMR experiments. We would also like to thank Jason Kaelber (Rutgers University) for help with cryoelectron microscopy measurements.
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  • 2399-3669
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