Graphene Biotransistor Interfaced with a Nitrifying Biofilm Public Deposited

http://ir.library.oregonstate.edu/concern/articles/3r0750594

This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society and can be found at:  http://pubs.acs.org/journal/estlcu

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  • Using a graphene field-effect transistor biosensor, we monitored the pH inside a living biofilm with fast temporal resolution (~ 1 s) over multi-hour time periods. The atomically-thin sensor is positioned between the biofilm and a supporting silicon oxide surface, providing non-invasive access to conditions at the base of the biofilm. We determine the transient changes in pH when the biofilm metabolizes substrate molecules and when it is exposed to biocide. The pH resolution is approximately 0.01 pH units when using 1-second time averaging. The sensor drift is approximately 0.01 pH units per hour. Our results demonstrate the potential of this technology to study biofilm metabolism and monitor biofilm health.
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  • Brown, M. A., Barker, L., Semprini, L., & Minot, E. D. (2015). Graphene Biotransistor Interfaced with a Nitrifying Biofilm. Environmental Science & Technology Letters, 2(4), 118-122. doi:10.1021/acs.estlett.5b00025
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