Graphene Biotransistor Interfaced with a Nitrifying Biofilm Public Deposited

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  • Using a graphene field-effect transistor biosensor, we monitored the pH inside a living biofilm with fast temporal resolution (~ 1 s) over multi-hour time periods. The atomically-thin sensor is positioned between the biofilm and a supporting silicon oxide surface, providing non-invasive access to conditions at the base of the biofilm. We determine the transient changes in pH when the biofilm metabolizes substrate molecules and when it is exposed to biocide. The pH resolution is approximately 0.01 pH units when using 1-second time averaging. The sensor drift is approximately 0.01 pH units per hour. Our results demonstrate the potential of this technology to study biofilm metabolism and monitor biofilm health.
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  • Brown, M. A., Barker, L., Semprini, L., & Minot, E. D. (2015). Graphene Biotransistor Interfaced with a Nitrifying Biofilm. Environmental Science & Technology Letters, 2(4), 118-122. doi:10.1021/acs.estlett.5b00025
Journal Title
Journal Volume
  • 2
Journal Issue/Number
  • 4
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Funding Statement (additional comments about funding)
  • Support for the study of N. europaea in biofilms was provided by a grant from the National Science Foundation’s Division of Chemical, Bioengineering, Environmental and Transport Systems Environmental Health and Safety of Nanotechnology program (no. 1067572). Development of the GFET biosensor was supported by the National Science Foundation under grant no. 1450967.
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