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| Abstract |
- The biogeochemical processes that occur in marine sediments on continental
margins are complex; however, from one perspective they can be considered with respect
to three geochemical zones based on the presence and form of methane: sulfate-methane
transition (SMTZ), gas hydrate stability zone (GHSZ), and free gas zone (FGZ). These
geochemical zones may harbor distinct microbial communities that are important in
biogeochemical carbon cycles. The objective of this study was to describe the microbial
communities in sediments from the SMTZ, GHSZ, and FGZ using molecular ecology
methods (i.e., PhyloChip microarray analysis and terminal restriction fragment length
polymorphism (T-RFLP)) and examining the results in the context of non-biological
parameters in the sediments. Non-metric multidimensional scaling and multi-response
permutation procedures were used to determine whether microbial community
compositions were significantly different in the three geochemical zones and to correlate
samples with abiotic characteristics of the sediments. This analysis indicated that
microbial communities from all three zones were distinct from one another and that
variables such as sulfate concentration, hydrate saturation of the nearest gas hydrate
layer, and depth (or unmeasured variables associated with depth e.g. temperature,
pressure) were correlated to differences between the three zones. The archaeal anaerobic
methanotrophs typically attributed to performing anaerobic oxidation of methane were
not detected in the SMTZ; however, the marine benthic group-B, which is often found in
SMTZ, was detected. Within the GHSZ, samples that were typically closer to layers that
contained higher hydrate saturation had indicator sequences related to Vibrio-type taxa.
These results suggest that the biogeographic patterns of microbial communities in marine
sediments are distinct based on geochemical zones defined by methane.
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| Citation |
- Briggs, B. B., Graw, M., Brodie, E. L., Bahk, J. J., Kim, S. H., Hyun, J. H., . . . Colwell, F. S. (2013). Microbial distributions detected by an oligonucleotide microarray across geochemical zones associated with methane in marine sediments from the Ulleung Basin. Marine and Petroleum Geology, 47, 147-154. doi:10.1016/j.marpetgeo.2013.02.015
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| Funding Statement (additional comments about funding) |
- Samples were collected as a part of the second Gas Hydrate Drilling Expedition in
the Ulleung Basin (UBGH2) in 2010 which was funded by the Ministry of Knowledge
Economy of Korea (MKE) under management of Korea Gas Hydrate Research and
Development Organization (KGHDO). We thank the entire UBGH2 scientific parties and
D/V Fugro Synergy crews for assisting us in the collection of samples. This project was
funded by the Department of Energy (DOE), National Energy Technology Laboratory, an
agency of the United States Government, through a support contract with URS Energy &
Construction, Inc. in support of the National Energy Laboratory’s ongoing research in
methane hydrates under the RES contract DE-FE0004000. Additional support came in
part by an appointment to the U.S. Department of Energy (DOE) Postgraduate Research
Program at the National Energy Technology Laboratory administered by the Oak Ridge
Institute for Science and Education and by the Center for Dark Energy Biosphere
Investigations (C-DEBI). Part of this work was performed at Lawrence Berkeley National
Lab under U.S. DOE contract number DE-AC02-05CH11232. Michael Graw was
supported under a Research Experience for Undergraduates (REU) program at Oregon
State University.
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| Additional Information |
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Previous issue date: 2013-11
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