Analysis of Genome Sequences from Plant Pathogenic Rhodococcus Reveals Genetic Novelties in Virulence Loci Public Deposited

Analytics
http://ir.library.oregonstate.edu/concern/articles/4m90f169p

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The article was published by the Public Library of Science and is in the public domain. The published article can be found at:  http://www.plosone.org/.

See related dataset: "Creason, Allison L.; Vandeputte, Olivier M.; Savory, Elizabeth A.; Davis II, Edward W.; Putnam, Melodie L.; Hu, Erdong; Swader-Hines, David; Mol, Adeline; Baucher, Marie; Prinsen, Els; Zdanowska, Magdalena; Givan, Scott A.; El Jaziri, Mondher; Loper, Joyce E.; Mahmud, Taifo; Chang, Jeff H. (2014): Analysis of genome sequences from plant pathogenic Rhodococcus reveals genetic novelties in virulence loci. Oregon State University Libraries. Dataset.  http://dx.doi.org/10.7267/N9PN93H8" at  http://hdl.handle.net/1957/48790

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Members of Gram-positive Actinobacteria cause economically important diseases to plants. Within the Rhodococcus genus, some members can cause growth deformities and persist as pathogens on a wide range of host plants. The current model predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with the fas operon central to virulence. The Fas proteins synthesize, modify, and activate a mixture of growth regulating cytokinins, which cause a hormonal imbalance in plants, resulting in abnormal growth. We sequenced and compared the genomes of 20 isolates of Rhodococcus to gain insights into the mechanisms and evolution of virulence in these bacteria. Horizontal gene transfer was identified as critical but limited in the scale of virulence evolution, as few loci are conserved and exclusive to phytopathogenic isolates. Although the fas operon is present in most phytopathogenic isolates, it is absent from phytopathogenic isolate A21d2. Instead, this isolate has a horizontally acquired gene chimera that encodes a novel fusion protein with isopentyltransferase and phosphoribohydrolase domains, predicted to be capable of catalyzing and activating cytokinins, respectively. Cytokinin profiling of the archetypal D188 isolate revealed only one activate cytokinin type that was specifically synthesized in a fas-dependent manner. These results suggest that only the isopentenyladenine cytokinin type is synthesized and necessary for Rhodococcus phytopathogenicity, which is not consistent with the extant model stating that a mixture of cytokinins is necessary for Rhodococcus to cause leafy gall symptoms. In all, data indicate that only four horizontally acquired functions are sufficient to confer the trait of phytopathogenicity to members of the genetically diverse clade of Rhodococcus.
License
Resource Type
DOI
Date Available
Date Issued
Citation
  • Creason AL, Vandeputte OM, Savory EA, Davis EW II, Putnam ML, et al. (2014) Analysis of Genome Sequences from Plant Pathogenic Rhodococcus Reveals Genetic Novelties in Virulence Loci. PLoS ONE 9(7): e101996. doi:10.1371/journal.pone.0101996
Series
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-08-08T20:41:20Z (GMT) No. of bitstreams: 3 license_rdf: 1089 bytes, checksum: 0a703d871bf062c5fdc7850b1496693b (MD5) CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences.pdf: 6116125 bytes, checksum: d2cf18140663a2ddce7d5122e29b4b17 (MD5) CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences_SupportingInformation.zip: 1566674 bytes, checksum: 0abfb1d1d2553fc56df576bdc6e930dd (MD5)
  • description.provenance : Made available in DSpace on 2014-08-08T20:41:20Z (GMT). No. of bitstreams: 3 license_rdf: 1089 bytes, checksum: 0a703d871bf062c5fdc7850b1496693b (MD5) CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences.pdf: 6116125 bytes, checksum: d2cf18140663a2ddce7d5122e29b4b17 (MD5) CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences_SupportingInformation.zip: 1566674 bytes, checksum: 0abfb1d1d2553fc56df576bdc6e930dd (MD5) Previous issue date: 2014-07-10
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-08-08T20:40:59Z No. of bitstreams: 3 license_rdf: 1089 bytes, checksum: 0a703d871bf062c5fdc7850b1496693b (MD5) CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences.pdf: 6116125 bytes, checksum: d2cf18140663a2ddce7d5122e29b4b17 (MD5) CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences_SupportingInformation.zip: 1566674 bytes, checksum: 0abfb1d1d2553fc56df576bdc6e930dd (MD5)

Relationships

Parents:

This work has no parents.

Last modified Default

Items

Thumbnail Title Date Uploaded Visibility Actions
3t945w79n?file=thumbnail CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences.pdf 11/16/2017 Public
Work CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences_SupportingInformation.zip 11/16/2017 Public