Article
 

The standalone aminopeptidase PepN catalyzes the maturation of blasticidin S from leucylblasticidin S

Public Deposited

Contenu téléchargeable

Télécharger le fichier PDF
https://ir.library.oregonstate.edu/concern/articles/5712m828g

Descriptions

Attribute NameValues
Creator
Abstract
  • The peptidyl nucleoside blasticidin S (BS) isolated from Streptomyces griseochromogenes was the first non-mercurial fungicide used on a large scale to prevent rice blast. In the biosynthesis of BS, leucylblasticidin S (LBS) was suggested as the penultimate metabolite with 20-fold less inhibitory activity than the final product BS. Incomplete conversion of LBS to BS at a variable efficiency ranging from 10% to 90% was observed either in the native strain S. griseochromogenes or a heterologous producer Streptomyces lividans WJ2. In this study, we determined that maturation of BS from LBS is not a spontaneous process but is governed by a standalone peptidase PepN, which hydrolyzes LBS in a pH-sensitive way with most appropriate of pH 7~8 but is inactive when the pH is below 5 or above 10. PepN1 and PepN2, two neighboring PepN homologs from Streptomyces lividans were purified in E. coli but displayed ca.100-fold difference in LBS hydrolytic activity. Overexpression of pepN1 in WJ2 enhanced BS yield by 100% and lowered the ratio of LBS to BS from 2:1 to 2:3. This work presents the expansion of the biological role for PepN in antibiotic maturation and the first report of hydrolysis of beta amide linkage by this conserved enzyme.
  • This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Nature Publishing Group. The published article can be found at: http://www.nature.com/articles/srep17641
Resource Type
DOI
Date Available
Date Issued
Citation
  • Yu, G., Li, L., Liu, X., Liu, G., Deng, Z., Zabriskie, M. T., ... & He, X. (2015). The standalone aminopeptidase PepN catalyzes the maturation of blasticidin S from leucylblasticidin S. Scientific Reports, 5, 17641. doi:10.1038/srep17641
Journal Title
Journal Volume
  • 5
Déclaration de droits
Funding Statement (additional comments about funding)
  • This work received support from the National Natural Science Foundation of China (31470195), 973 program from the Ministry of Science and Technology (2012CB721004).
Publisher
Peer Reviewed
Language
Replaces

Des relations

Parents:

This work has no parents.

Articles

La vignette Titre Date de téléchargement Visibilité actes
file details ZabriskieMarkPharmacyStandaloneAminopeptidasePepN.pdf 07/26/2017 Public
file details ZabriskieMarkPharmacyStandaloneAminopeptidasePepN(SupplementaryMaterial).pdf 07/26/2017 Public