Developing and interpreting aqueous functional assays for comparative property-activity relationships of different nanoparticles Public Deposited

Downloadable Content

Download PDF


Attribute NameValues
  • It is difficult to relate intrinsic nanomaterial properties to their functional behavior in the environment. Unlike frameworks for dissolved organic chemicals, there are few frameworks comparing multiple and inter-related properties of engineered nanomaterials (ENMs) to their fate, exposure, and hazard in environmental systems. We developed and evaluated reproducibility and inter-correlation of 12 physical, chemical, and biological functional assays in water for eight different engineered nanomaterials (ENMs) and interpreted results using activity-profiling radar plots. The functional assays were highly reproducible when run in triplicate (average coefficient of variation [CV] = 6.6%). Radar plots showed that each nanomaterial exhibited unique activity profiles. Reactivity assays showed dissolution or aggregation potential for some ENMs. Surprisingly, multi-walled carbon nanotubes (MWCNTs) exhibited movement in a magnetic field. We found high inter-correlations between cloud point extraction (CPE) and distribution to sewage sludge (R-2 = 0.99), dissolution at pH 8 and pH 4.9 (R-2 = 0.98), and dissolution at pH 8 and zebrafish mortality at 24 hpf (R-2 = 0.94). Additionally, most ENMs tend to distribute out of water and into other phases (i.e., soil surfaces, surfactant micelles, and sewage sludge). The activity-profiling radar plots provide a framework and estimations of likely ENM disposition in the environment. (C) 2018 Elsevier B.V. All rights reserved.
Resource Type
Date Issued
Journal Title
Journal Volume
  • 628-629
Rights Statement
  • 0048-9697



This work has no parents.