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ECD of Tyrosine Phosphorylation in a Triple Quadrupole Mass Spectrometer with a Radio-Frequency-Free Electromagnetostatic Cell

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Abstract
  • A radio frequency-free electromagnetostatic (EMS) cell devised for electron-capture dissociation (ECD) of ions has been retrofitted into the collision-induced dissociation (CID) section of a triple quadrupole mass spectrometer to enable recording of ECD product-ion mass spectra and simultaneous recording of ECD-CID product-ion mass spectra. This modified instrument can be used to produce easily interpretable ECD and ECD-CID product-ion mass spectra of tyrosine-phosphorylated peptides that cover over 50% of their respective amino-acid sequences and readily identify their respective sites of phosphorylation. ECD fragmentation of doubly protonated, tyrosine-phosphorylated peptides, which was difficult to observe with FT-ICR instruments, occurs efficiently in the EMS cell.
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  • Voinov, V. G., Bennett, S. E., Beckman, J. S., & Barofsky, D. F. (2014). ECD of Tyrosine Phosphorylation in a Triple Quadrupole Mass Spectrometer with a Radio-Frequency-Free Electromagnetostatic Cell. Journal of The American Society for Mass Spectrometry, 25(10), 1730-1738. doi:10.1007/s13361-014-0956-2
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  • 25
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  • 10
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  • Various aspects of this research were supported by grants from the NSF (CHE-0924027), the Oregon Nanoscience and Microtechnologies Institute (#09-31 #3.5), NIH NCRR (R01RR026275), NIH NIEHS (ES00210–Environmental Health Sciences Center), and Agilent Technologies (#2443).
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