Development of an improved extraction and HPLC method for the measurement of ascorbic acid in cows' milk from processing plants and retail outlets Public Deposited

http://ir.library.oregonstate.edu/concern/articles/73666930h

This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by John Wiley & Sons, Inc. and can be found at:  http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291365-2621.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • An improved extraction (2.5% HPO₃, 5 mM dithiothreitol) and HPLC quantification using a C-18 column at 35°C and 0.1M acetic acid (98%) and acetonitrile (2%) mobile phase was developed to quantify total ascorbic acid (AA) in commercial whole/semi-skim/skim raw/pasteurized/UHT milk packaged in opaque bags, transparent plastic, cardboard, and Tetra Brik™. AA content ranged 0.21-10 and 3.4-16 mg/L in milk from retail outlets and processing plants, respectively, and was higher in organic milk. For same processor/lot samples, pasteurized milk showed higher AA content than UHT milk. This was not true for retail outlets samples. AA content was similar for whole/semi-skim and semi-skim/skim milk but not for whole/skim comparisons. Among UHT samples, the AA content trend was whole<semi-skim<skim and lower for UHT milk in opaque plastic and Tetra Brik™ container. After 14d at 4°C in the dark, AA losses ranged 35-83% depending on milk type and preservation method with a higher AA retention in unopened containers.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Chotyakul, N., Pateiro-Moure, M., Martínez-Carballo, E., Saraiva, J. A., Torres, J. A. and Pérez-Lamela, C. (2014), Development of an improved extraction and HPLC method for the measurement of ascorbic acid in cows' milk from processing plants and retail outlets. International Journal of Food Science & Technology, 49(3), 679–688. doi: 10.1111/ijfs.12350
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items