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Triclosan Exposure Is Associated with Rapid Restructuring of the Microbiome in Adult Zebrafish Public Deposited

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https://ir.library.oregonstate.edu/concern/articles/7d278v68f

Data Availability Statement: All sequencing data files are available from the SRA database (accession number PRJNA313944).

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Abstract
  • Growing evidence indicates that disrupting the microbial community that comprises the intestinal tract, known as the gut microbiome, can contribute to the development or severity of disease. As a result, it is important to discern the agents responsible for microbiome disruption. While animals are frequently exposed to a diverse array of environmental chemicals, little is known about their effects on gut microbiome stability and structure. Here, we demonstrate how zebrafish can be used to glean insight into the effects of environmental chemical exposure on the structure and ecological dynamics of the gut microbiome. Specifically, we exposed forty-five adult zebrafish to triclosan-laden food for four or seven days or a control diet, and analyzed their microbial communities using 16S rRNA amplicon sequencing. Triclosan exposure was associated with rapid shifts in microbiome structure and diversity. We find evidence that several operational taxonomic units (OTUs) associated with the family Enterobacteriaceae appear to be susceptible to triclosan exposure, while OTUs associated with the genus Pseudomonas appeared to be more resilient and resistant to exposure. We also found that triclosan exposure is associated with topological alterations to microbial interaction networks and results in an overall increase in the number of negative interactions per microbe in these networks. Together these data indicate that triclosan exposure results in altered composition and ecological dynamics of microbial communities in the gut. Our work demonstrates that because zebrafish afford rapid and inexpensive interrogation of a large number of individuals, it is a useful experimental system for the discovery of the gut microbiome’s interaction with environmental chemicals.
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  • Gaulke, C. A., Barton, C. L., Proffitt, S., Tanguay, R. L., & Sharpton, T. J. (2016). Triclosan Exposure is Associated with Rapid Restructuring of the Microbiome in Adult Zebrafish. PLoS ONE, 11(5), e0154632. doi:10.1371/journal.pone.0154632
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  • 11
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  • 5
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  • Funding for this work was made available by National Institute of Environmental Health Sciences supported Environmental Health Sciences Center P30 ES000210 and a pilot grant to TJS, and institutional funds to TJS.
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  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-06-23T14:06:47Z No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) GaulkeTriclosanExposureAssociated.PDF: 2270185 bytes, checksum: 46230151a4f96471f7ba4cf07ee1c6cc (MD5) GaulkeTriclosanExposureAssociatedS1-S3Files.zip: 1341719 bytes, checksum: 1724bf43e5382899cc3f5cec2aae09bf (MD5)
  • description.provenance : Made available in DSpace on 2016-06-23T14:07:31Z (GMT). No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) GaulkeTriclosanExposureAssociated.PDF: 2270185 bytes, checksum: 46230151a4f96471f7ba4cf07ee1c6cc (MD5) GaulkeTriclosanExposureAssociatedS1-S3Files.zip: 1341719 bytes, checksum: 1724bf43e5382899cc3f5cec2aae09bf (MD5) Previous issue date: 2016-05-18
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-06-23T14:07:31Z (GMT) No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) GaulkeTriclosanExposureAssociated.PDF: 2270185 bytes, checksum: 46230151a4f96471f7ba4cf07ee1c6cc (MD5) GaulkeTriclosanExposureAssociatedS1-S3Files.zip: 1341719 bytes, checksum: 1724bf43e5382899cc3f5cec2aae09bf (MD5)

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