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The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth of Erwinia amylovora and acts as a seed germination-arrest factor Public Deposited

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  • The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) shares biological activities with 4-formylaminooxyvinylglycine, a related molecule produced by Pseudomonas fluorescens WH6. We found that culture filtrates of a P.aeruginosa strain overproducing AMB weakly interfered with seed germination of the grassy weed Poa annua and strongly inhibited growth of Erwinia amylovora, the causal agent of the devastating orchard crop disease known as fire blight. AMB was active against a 4-formylaminooxyvinylglycine-resistant isolate of E.amylovora, suggesting that the molecular targets of the two oxyvinylglycines in Erwinia do not, or not entirely, overlap. The AMB biosynthesis and transport genes were shown to be organized in two separate transcriptional units, ambA and ambBCDE, which were successfully expressed from IPTG-inducible tac promoters in the heterologous host P.fluorescens CHA0. Engineered AMB production enabled this model biocontrol strain to become inhibitory against E.amylovora and to weakly interfere with the germination of several graminaceous seeds. We conclude that AMB production requires no additional genes besides ambABCDE and we speculate that their expression in marketed fire blight biocontrol strains could potentially contribute to disease control.
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  • Lee, X., Azevedo, M. D., Armstrong, D. J., Banowetz, G. M. and Reimmann, C. (2013), The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth of Erwinia amylovora and acts as a seed germination-arrest factor. Environmental Microbiology Reports, 5: 83–89. doi: 10.1111/j.1758-2229.2012.00395.x
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  • 5
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  • At Oregon State University, support from the USDA-CSREES Grass Seed Cropping Systems for Sustainable Agriculture Special Grant Program and from the OSU Agricultural Research Foundation is gratefully acknowledged
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  • description.provenance : Submitted by Deborah Campbell (deborah.campbell@oregonstate.edu) on 2013-03-11T17:17:46Z No. of bitstreams: 1 ArmstrongDonaldJBotanyPlantPathologyPseudomonasAeruginosaAntimetabolite.pdf: 705866 bytes, checksum: 4e7f86fc97fb9dd48528ff34476b8915 (MD5)
  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-03-11T17:49:13Z (GMT) No. of bitstreams: 1 ArmstrongDonaldJBotanyPlantPathologyPseudomonasAeruginosaAntimetabolite.pdf: 705866 bytes, checksum: 4e7f86fc97fb9dd48528ff34476b8915 (MD5)
  • description.provenance : Made available in DSpace on 2013-03-11T17:49:13Z (GMT). No. of bitstreams: 1 ArmstrongDonaldJBotanyPlantPathologyPseudomonasAeruginosaAntimetabolite.pdf: 705866 bytes, checksum: 4e7f86fc97fb9dd48528ff34476b8915 (MD5) Previous issue date: 2012-10-08

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