Article

 

The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth of Erwinia amylovora and acts as a seed germination-arrest factor Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/articles/7w62f8943

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. The published article is copyrighted by Blackwell Publishing Ltd. and can be found at:  http://onlinelibrary.wiley.com/ and Society for Applied Microbiology ( http://www.sfam.org.uk/).

Descriptions

Attribute NameValues
Creator
Abstract
  • The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) shares biological activities with 4-formylaminooxyvinylglycine, a related molecule produced by Pseudomonas fluorescens WH6. We found that culture filtrates of a P.aeruginosa strain overproducing AMB weakly interfered with seed germination of the grassy weed Poa annua and strongly inhibited growth of Erwinia amylovora, the causal agent of the devastating orchard crop disease known as fire blight. AMB was active against a 4-formylaminooxyvinylglycine-resistant isolate of E.amylovora, suggesting that the molecular targets of the two oxyvinylglycines in Erwinia do not, or not entirely, overlap. The AMB biosynthesis and transport genes were shown to be organized in two separate transcriptional units, ambA and ambBCDE, which were successfully expressed from IPTG-inducible tac promoters in the heterologous host P.fluorescens CHA0. Engineered AMB production enabled this model biocontrol strain to become inhibitory against E.amylovora and to weakly interfere with the germination of several graminaceous seeds. We conclude that AMB production requires no additional genes besides ambABCDE and we speculate that their expression in marketed fire blight biocontrol strains could potentially contribute to disease control.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Lee, X., Azevedo, M. D., Armstrong, D. J., Banowetz, G. M. and Reimmann, C. (2013), The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth of Erwinia amylovora and acts as a seed germination-arrest factor. Environmental Microbiology Reports, 5: 83–89. doi: 10.1111/j.1758-2229.2012.00395.x
Journal Title
Journal Volume
  • 5
Journal Issue/Number
  • 1
Academic Affiliation
Keyword
Rights Statement
Funding Statement (additional comments about funding)
  • At Oregon State University, support from the USDA-CSREES Grass Seed Cropping Systems for Sustainable Agriculture Special Grant Program and from the OSU Agricultural Research Foundation is gratefully acknowledged
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Deborah Campbell (deborah.campbell@oregonstate.edu) on 2013-03-11T17:17:46Z No. of bitstreams: 1 ArmstrongDonaldJBotanyPlantPathologyPseudomonasAeruginosaAntimetabolite.pdf: 705866 bytes, checksum: 4e7f86fc97fb9dd48528ff34476b8915 (MD5)
  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-03-11T17:49:13Z (GMT) No. of bitstreams: 1 ArmstrongDonaldJBotanyPlantPathologyPseudomonasAeruginosaAntimetabolite.pdf: 705866 bytes, checksum: 4e7f86fc97fb9dd48528ff34476b8915 (MD5)
  • description.provenance : Made available in DSpace on 2013-03-11T17:49:13Z (GMT). No. of bitstreams: 1 ArmstrongDonaldJBotanyPlantPathologyPseudomonasAeruginosaAntimetabolite.pdf: 705866 bytes, checksum: 4e7f86fc97fb9dd48528ff34476b8915 (MD5) Previous issue date: 2012-10-08

Relationships

Parents:

This work has no parents.

Items