Deficiency of CCAAT/Enhancer Binding Protein-Epsilon Reduces Atherosclerotic Lesions in LDLR-/- Mice Public Deposited

http://ir.library.oregonstate.edu/concern/articles/9593tw85t

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  • The CCAAT/enhancer binding proteins (C/EBPs) are transcription factors involved in hematopoietic cell development and induction of several inflammatory mediators. C/EBPε is expressed only in myeloid cells including monocytes/macrophages. Atherosclerosis is an inflammatory disorder of the vascular wall and circulating immune cells such as monocytes/macrophages. Mice deficient in the low density lipoprotein (LDL) receptor (Ldlr-/-) fed on a high cholesterol diet (HCD) show elevated blood cholesterol levels and are widely used as models to study human atherosclerosis. In this study, we generated Ldlr and Cebpe double-knockout (llee) mice and compared their atherogenic phenotypes to Ldlr single deficient (llEE) mice after HCD. Macrophages from llee mice have reduced lipid uptake by foam cells and impaired phagokinetic motility in vitro compared to macrophages from llEE mice. Also, compared to llEE mice, llee mice have alterations of lipid metabolism, and reduced atheroma and obesity, particularly the males. Peritoneal macrophages of llee male mice have reduced mRNA expression of FABP4, a fatty acid binding protein implicated in atherosclerosis. Overall, our study suggests that the myeloid specific factor C/EBPε is involved in systemic lipid metabolism and that silencing of C/EBPε could decrease the development of atherosclerosis.
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  • Okamoto R, Gery S, Gombart AF, Wang X, Castellani LW, et al. (2014) Deficiency of CCAAT/Enhancer Binding Protein-Epsilon Reduces Atherosclerotic Lesions in LDLR-/- Mice. PLoS ONE 9(1): e85341. doi:10.1371/journal.pone.0085341
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  • description.provenance : Made available in DSpace on 2014-04-09T22:11:46Z (GMT). No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) GombartAdrianBiochemistryBiophysicsDeficiencyCCAATEnhancer.pdf: 2712657 bytes, checksum: 06915a3999580723684d1b9fbb167470 (MD5) GombartAdrianBiochemistryBiophysicsDeficiencyCCAATEnhancer_SupportingInformation.zip: 1465171 bytes, checksum: 8b2d14ed4f3d5bd14160140fa6b91497 (MD5) Previous issue date: 2014-01-28
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  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-04-09T22:11:33Z No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) GombartAdrianBiochemistryBiophysicsDeficiencyCCAATEnhancer.pdf: 2712657 bytes, checksum: 06915a3999580723684d1b9fbb167470 (MD5) GombartAdrianBiochemistryBiophysicsDeficiencyCCAATEnhancer_SupportingInformation.zip: 1465171 bytes, checksum: 8b2d14ed4f3d5bd14160140fa6b91497 (MD5)

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