Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency Public Deposited

http://ir.library.oregonstate.edu/concern/articles/9w0325015

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  • Cyprinid herpesvirus 3 (CyHV-3), commonly known as koi herpesvirus (KHV), is a member of the Alloherpesviridae, and is a recently discovered emerging herpesvirus that is highly pathogenic for koi and common carp. Our previous study demonstrated that CyHV-3 becomes latent in peripheral white blood cells (WBC). In this study, CyHV-3 latency was further investigated in IgM⁺ WBC. The presence of the CyHV-3 genome in IgM⁺ WBC was about 20-fold greater than in IgM⁻ WBC. To determine whether CyHV-3 expressed genes during latency, transcription from all eight open reading frames (ORFs) in the terminal repeat was investigated in IgM⁺ WBC from koi with latent CyHV-3 infection. Only a spliced ORF6 transcript was found to be abundantly expressed in IgM⁺ WBC from CyHV-3 latently infected koi. The spliced ORF6 transcript was also detected in vitro during productive infection as early as 1 day postinfection. The ORF6 transcript from in vitro infection begins at -127 bp upstream of the ATG codon and ends +188 bp downstream of the stop codon, +20 bp downstream of the polyadenylation signal. The hypothetical protein of ORF6 contains a consensus sequence with homology to a conserved domain of EBNA-3B and ICP4 from Epstein-Barr virus and herpes simplex virus 1, respectively, both members of the Herpesviridae. This is the first report of latent CyHV-3 in B cells and identification of gene transcription during latency for a member of the Alloherpesviridae.
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  • Reed, A. N., Izume, S., Dolan, B. P., LaPatra, S., Kent, M., Dong, J., & Jin, L. (2014). Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency. Journal of Virology, 88(16), 9297-9309. doi:10.1128/JVI.00990-14
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  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-10-20T16:57:20Z No. of bitstreams: 1 ReedAimeeVetMedIdentificationBCells.pdf: 2729789 bytes, checksum: 152b9811da589b481f49ac5deaa7a1e1 (MD5)
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-10-20T16:57:31Z (GMT) No. of bitstreams: 1 ReedAimeeVetMedIdentificationBCells.pdf: 2729789 bytes, checksum: 152b9811da589b481f49ac5deaa7a1e1 (MD5)
  • description.provenance : Made available in DSpace on 2014-10-20T16:57:31Z (GMT). No. of bitstreams: 1 ReedAimeeVetMedIdentificationBCells.pdf: 2729789 bytes, checksum: 152b9811da589b481f49ac5deaa7a1e1 (MD5) Previous issue date: 2014-06-04

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