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Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency

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  • Cyprinid herpesvirus 3 (CyHV-3), commonly known as koi herpesvirus (KHV), is a member of the Alloherpesviridae, and is a recently discovered emerging herpesvirus that is highly pathogenic for koi and common carp. Our previous study demonstrated that CyHV-3 becomes latent in peripheral white blood cells (WBC). In this study, CyHV-3 latency was further investigated in IgM⁺ WBC. The presence of the CyHV-3 genome in IgM⁺ WBC was about 20-fold greater than in IgM⁻ WBC. To determine whether CyHV-3 expressed genes during latency, transcription from all eight open reading frames (ORFs) in the terminal repeat was investigated in IgM⁺ WBC from koi with latent CyHV-3 infection. Only a spliced ORF6 transcript was found to be abundantly expressed in IgM⁺ WBC from CyHV-3 latently infected koi. The spliced ORF6 transcript was also detected in vitro during productive infection as early as 1 day postinfection. The ORF6 transcript from in vitro infection begins at -127 bp upstream of the ATG codon and ends +188 bp downstream of the stop codon, +20 bp downstream of the polyadenylation signal. The hypothetical protein of ORF6 contains a consensus sequence with homology to a conserved domain of EBNA-3B and ICP4 from Epstein-Barr virus and herpes simplex virus 1, respectively, both members of the Herpesviridae. This is the first report of latent CyHV-3 in B cells and identification of gene transcription during latency for a member of the Alloherpesviridae.
  • This is the publisher’s final pdf. The published article is copyrighted by the American Society for Microbiology and can be found at: http://jvi.asm.org/.
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  • Reed, A. N., Izume, S., Dolan, B. P., LaPatra, S., Kent, M., Dong, J., & Jin, L. (2014). Identification of B Cells as a Major Site for Cyprinid Herpesvirus 3 Latency. Journal of Virology, 88(16), 9297-9309. doi:10.1128/JVI.00990-14
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  • 88
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  • 16
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  • We thank the American Koi Club Association for funding this study. A. N.Reed was supported by Office of the Director of the National Institutes of Health T32 training grant RR023917 and grant T32OD011020.
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