Article
 

Bovine hepatic differential gene expression in response to perennial ryegrass staggers

Public Deposited

Contenu téléchargeable

Télécharger le fichier PDF
https://ir.library.oregonstate.edu/concern/articles/bc386k98f

Descriptions

Attribute NameValues
Creator
Abstract
  • “Perennial ryegrass staggers” is a neurological condition characterized by muscular tremors and ataxia in livestock that ingest the indole diterpene lolitrem B from endophyte-infected perennial ryegrass. While the neurotoxic mechanism of action of ryegrass staggers has been defined, little is known about metabolic and other molecular processes that lolitrem B may affect in livestock. The objective of this study was to characterize differential gene expression in the liver of animals fed lolitrem B-containing feed over an extended period of time. Eighteen steers were fed one of three rations (n =6/group) containing lolitrem B (247, 1,554 or 2,256 parts per billion (ppb, DM basis)) over 64 days in a double-blind study. Microarray and confirmatory qPCR were performed to evaluate the hepatic gene expression profile from biopsies taken on days 0 and 64. Widespread perturbation of gene expression was observed in all groups receiving lolitrem B, with a total of 152 differential genes identified (FDR ≤ 0.05). This suggests that chronic exposure to lolitrem B, even at levels below the current threshold of toxicity (2,000 ppb LB), can perturb many genes, biological processes and pathways. Gene ontology (GO) and Kyoto Encyclopedia of Genes and genomes (KEGG) pathway analyses indicated that many of these genes were categorized under lipid/steroid biosynthesis/metabolism and oxidation-reduction. Specifically, genes involved in the biosynthesis pathway of ceramide, a sphingolipid molecule (ACSS2, LASS6 and SCD) and changes in neurosignaling through alteration of nitric oxide synthase activity (ARG1 and GPX4) were up-regulated. Future work should focus on the overall balance between ceramide and its metabolites and antioxidants/oxidants in a variety of body matrices in animals with perennial ryegrass staggers, to determine how these compounds contribute to the overall etiology of this disease.
  • This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wageningen Academic Publishers. The original publication is available at: http://www.wageningenacademic.com/doi/10.3920/WMJ2014.1749
  • Keywords: microarray, lolitrem B, cattle, ceramide
Resource Type
DOI
Date Available
Date Issued
Citation
  • Li, L., Tanaree, D. D., Di, Y., Estill, C. T., Duringer, J. M., Blythe, L. L., ... & Craig, A. M. (2015). Bovine hepatic differential gene expression in response to perennial ryegrass staggers. World Mycotoxin Journal, 8(3), 351-360. doi:10.3920/WMJ2014.1749
Journal Title
Journal Volume
  • 8
Journal Issue/Number
  • 3
Déclaration de droits
Funding Statement (additional comments about funding)
  • This work was supported by the Oregon Agricultural Experiment Station (ORE00871) and USDA, Agriculture Research Service project 58-6227-8-044.
Publisher
Peer Reviewed
Language
Replaces

Des relations

Parents:

This work has no parents.

Articles

La vignette Titre Date de téléchargement Visibilité actes
file details CraigAMorrieVeterinaryMedicineBovineHepaticDifferentialGeneExpression.pdf 2017-07-26 Public Télécharger