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Small RNA binding is a common strategy to suppress RNA silencing by several viral suppressors Public Deposited

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https://ir.library.oregonstate.edu/concern/articles/br86b434w

Originally published in the EMBO Journal:  http://www.nature.com/emboj/index.html.

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  • RNA silencing is an evolutionarily conserved system that functions as an antiviral mechanism in higher plants and insects. To counteract RNA silencing, viruses express silencing suppressors that interfere with both siRNA- and microRNA-guided silencing pathways. We used comparative in vitro and in vivo approaches to analyse the molecular mechanism of suppression by three well-studied silencing suppressors. We found that silencing suppressors p19, p21 and HC-Pro each inhibit the intermediate step of RNA silencing via binding to siRNAs, although the molecular features required for duplex siRNA binding differ among the three proteins. None of the suppressors affected the activity of preassembled RISC complexes. In contrast, each suppressor uniformly inhibited the siRNA-initiated RISC assembly pathway by preventing RNA silencing initiator complex formation
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  • Lakatos, L., Csorba, T., Pantaleo, V., Chapman, E. J., Carrington, J. C., Liu, Y. P., Dolja, V. V., Calvino, L. F., López-Moya, J. J., & Burgyán, J. (2006). Small RNA binding is a common strategy to suppress RNA silencing by several viral suppressors. The EMBO journal, 25(12), 2768-2780
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  • We are grateful to Olivier Voinnet for providing the miR171.1 and miR171.2 sensor constructs. We also thank David Baulcombe for the GFP16c/RDR6i line. We are also grateful to Ga´bor Giczey for corrections. This research was supported by grants from the Hungarian Scientific Research Fund (OTKA; T046728 and OTKA; T048852), the ‘RIBOREG’ EU project to (LSHG-CT-2003503022), the Scientia Amabilis Foundation. Work in JCC’s lab was supported by grants from the National Science Foundation (MCB-0209836), National Institutes of Health (AI43288) and US Department of Agriculture (2005–35319-15280). Work in VVD’s lab is supported in part by a grant from the National Institutes of Health (GM053190). LL is a recipient of a Bolyai Ja´nos Fellowship. VP was a recipient of CNR-NATO fellowship Programs n.215.35 and 217.35
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