Article

 

Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows Public Deposited

Analytics

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/articles/br86b537x

This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by BioMed Central . The published article can be found at:  http://jasbsci.biomedcentral.com/

Descriptions

Attribute NameValues
Creator
Abstract
  • Background: Main objectives were to determine to what extent Smartamine M (SM) supplementation to a prepartal higher-energy diet could alter neutrophil (PMN) and liver tissue immunometabolic biomarkers, and whether those responses were comparable to those in cows fed a prepartal lower-energy diet (CON). Results: Twenty-eight multiparous Holstein cows were fed CON (NE[subscript]L = 1.24 Mcal/kg DM) during d −50 to d −22 relative to calving. From d −21 to calving, cows were randomly assigned to a higher-energy diet (OVE, n = 9; NE[subscript]L = 1.54 Mcal/kg DM), OVE plus SM (OVE + SM, n = 10; SM= 0.07 % of DM) or remained on CON (n = 9). All cows received the same basal lactation diet (NE[subscript]L = 1.75 Mcal/kg DM). Supplementation of SM (OVE + SM) continued until 30 d postpartum. Liver biopsies were harvested at d −10, 7, and 21 relative to parturition. Blood PMN isolated at −10, 3, and 21 d relative to calving was used to evaluate gene expression. As expected, OVE increased liver lipid content postpartum; however, cows fed OVE + SM or CON had lower concentrations than OVE. Compared with OVE, cows in CON and OVE + SM had greater DMI postpartum and milk production. Furthermore, cows fed OVE + SM had the greatest milk protein and fat percentage and lowest milk SCC despite having intermediate PMN phagocytic capacity. Adaptations in PMN gene expression in OVE + SM cows associated with the lower SCC were gradual increases from −10 to 21 d in genes that facilitate migration into inflammatory sites (SELL, ITGAM), enzymes essential for reducing reactive oxygen metabolites (SOD1, SOD2), and a transcription factor(s) required for controlling PMN development (RXRA). The greater expression of TLR4 on d 3, key for activation of innate immunity due to inflammation, in OVE compared with CON cows suggests a more pronounced inflammatory state. Feeding OVE + SM dampened the upregulation of TLR4, despite the fact that these cows had similar expression of the pro-inflammatory genes NFKB1 and TNF as OVE. Cows in CON had lower overall expression of these inflammation-related genes and GSR, which generates reduced glutathione, an important cellular antioxidant. Conclusions: Although CON cows appeared to have a less stressful transition into lactation, SM supplementation was effective in alleviating negative effects of energy-overfeeding. As such, SM was beneficial in terms of production and appeared to boost the response of PMN in a way that improved overall cow health.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Li, C., Batistel, F., Osorio, J. S., Drackley, J. K., Luchini, D., & Loor, J. J. (2016). Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows. Journal of Animal Science and Biotechnology, 7, 18. doi:10.1186/s40104-016-0077-9
Journal Title
Journal Volume
  • 7
Keyword
Rights Statement
Funding Statement (additional comments about funding)
  • Financial support for the research was provided in part by Adisseo (Commentary, France) and Hatch funds under project ILLU-538-914, National Institute of Food and Agriculture, Washington, DC, USA.
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-04-15T17:46:09Z No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) LiPeripartalRumenProtected.pdf: 1240838 bytes, checksum: f84896989f779cf0367704b0bc6c87a7 (MD5) LiPeripartalRumenProtectedTablesS1-S5.pdf: 113793 bytes, checksum: 5b630ef9e868bdafb8c14ccb2eb5ae69 (MD5)
  • description.provenance : Made available in DSpace on 2016-04-15T17:46:56Z (GMT). No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) LiPeripartalRumenProtected.pdf: 1240838 bytes, checksum: f84896989f779cf0367704b0bc6c87a7 (MD5) LiPeripartalRumenProtectedTablesS1-S5.pdf: 113793 bytes, checksum: 5b630ef9e868bdafb8c14ccb2eb5ae69 (MD5) Previous issue date: 2016-03-09
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-04-15T17:46:56Z (GMT) No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) LiPeripartalRumenProtected.pdf: 1240838 bytes, checksum: f84896989f779cf0367704b0bc6c87a7 (MD5) LiPeripartalRumenProtectedTablesS1-S5.pdf: 113793 bytes, checksum: 5b630ef9e868bdafb8c14ccb2eb5ae69 (MD5)

Relationships

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
LiPeripartalRumenProtected.pdf 07/26/2017 Public
LiPeripartalRumenProtectedTablesS1-S5.pdf 07/26/2017 Public