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Astragaloside IV Inhibits NF-κB Activation and Inflammatory Gene Expression in LPS-Treated Mice Public Deposited

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  • In this study we investigated the role of astragaloside IV (AS-IV), one of the major active constituents purified from the Chinese medicinal herb Astragalus membranaceus, in LPS-induced acute inflammatory responses in mice in vivo and examined possible underlying mechanisms. Mice were assigned to four groups: vehicle-treated control animals; AS-IV-treated animals (10 mg/kg b.w. AS-IV daily i.p. injection for 6 days); LPS-treated animals; and AS-IV plus LPS-treated animals. We found that AS-IV treatment significantly inhibited LPS-induced increases in serum levels of MCP-1 and TNF by 82% and 49%, respectively. AS-IV also inhibited LPS-induced up regulation of inflammatory gene expression in different organs. Lung mRNA levels of cellular adhesion molecules, MCP-1, TNF𝛼, IL-6, and TLR4 were significantly attenuated, and lung neutrophil infiltration and activation were strongly inhibited, as reflected by decreased myeloperoxidase content, when the mice were pretreated with AS-IV. Similar results were observed in heart, aorta, kidney, and liver. Furthermore, AS-IV significantly suppressed LPS-induced NF-𝜅B and AP-1DNA-binding activities in lung and heart. In conclusion, our data provide new in vivo evidence that AS-IV effectively inhibits LPS-induced acute inflammatory responses by modulating NF-𝜅B and AP-1 signaling pathways. Our results suggest that AS-IV may be useful for the prevention or treatment of inflammatory diseases.
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  • Zhang, W. J., & Frei, B. (2015). Astragaloside IV Inhibits NF-κB Activation and Inflammatory Gene Expression in LPS-Treated Mice. Mediators of Inflammation, 2015, 274314. doi:10.1155/2015/274314
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  • This work was supported by NCCAM Center of Excellence Grant P01 AT002034 from the National Institutes of Health.
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  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2015-05-21T15:41:45Z (GMT) No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ZhangWeijianBiochemBiophysAstragalosideInhibits.pdf: 1318682 bytes, checksum: 86b68ef754d1b061f2ecc224c36c3dfe (MD5)
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2015-05-21T15:40:52Z No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ZhangWeijianBiochemBiophysAstragalosideInhibits.pdf: 1318682 bytes, checksum: 86b68ef754d1b061f2ecc224c36c3dfe (MD5)
  • description.provenance : Made available in DSpace on 2015-05-21T15:41:45Z (GMT). No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ZhangWeijianBiochemBiophysAstragalosideInhibits.pdf: 1318682 bytes, checksum: 86b68ef754d1b061f2ecc224c36c3dfe (MD5) Previous issue date: 2015

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