Robust enzymatic saccharification of a Douglas-fir forest harvest residue by SPORL Public Deposited

http://ir.library.oregonstate.edu/concern/articles/fx719p37h

To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by Elsevier and can be found at:  http://www.journals.elsevier.com/biomass-and-bioenergy/.

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  • Forest harvest residues can be a cost-effective feedstock for a biorefinery, but the high lignin content of forest residues is a major barrier for enzymatic sugar production. Sulfite pretreatment to overcome strong recalcitrance of lignocelluloses (SPORL) was applied to a Douglas-fir (Pseudotsuga menziesii (Mirb) Franco var. menziesii) forest residue in a range of sulfite and acid loadings at 165°C for 75 min with liquid to wood ratio of 3:1. Sodium bisulfite and sulfuric acid charge as mass fraction of oven dry biomass of 12% and 2.21%, respectively, was optimal in terms of enzymatic cellulose saccharification, sugar yield and formation of hydroxymethylfurfural (HMF) and furfural. Enzymatic glucose yield was 345 g kg⁻¹, or equivalent to 82.3% of theoretical at a cellulase (CTec2) dosage of 15 filter paper unit (FPU) per gram of glucan. HMF and furfural formation were low at approximately 2.5 g L⁻¹ each in the pretreatment hydrolyzate. Delignification was important to achieve good cellulose saccharification efficiency, however, approximately 80-90% hemicellulose removal is also required. Substrate enzymatic digestibility (SED) was found to correlate to a combined parameter Z(CHF) of delignification and hemicellulose dissolution well, suggesting that the combined hydrolysis factor (CHF) - a pretreatment severity measure - can be used to predict saccharification of forest residue for scale-up studies to reduce numbers of experiments.
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  • Leu, S. Y., Zhu, J. Y., Gleisner, R., Sessions, J., & Marrs, G. (2013). Robust enzymatic saccharification of a Douglas-fir forest harvest residue by SPORL. Biomass and Bioenergy, 59, 393-401. doi:10.1016/j.biombioe.2013.08.014
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