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Development and Characterization of Monoclonal Antibodies against the Snakehead Rhabdovirus Public Deposited

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  • Monoclonal antibodies (MAbs) directed against the snakehead rhabdovirus (SHRV) were produced. These MAbs were characterized by immunofluorescence and neutralization tests, and by their ability to immunoprecipitate viral proteins. Of 15 MAbs developed, 9 were isotyped as IgG1 and 6 were IgG2a. Eight of the MAbs recognized the viral glycoprotein in an immuneprecipitation assay. Three of these, designated E1-9A, P10C, and O10F, had neutralizing activity. By immunofluorescence, 12 MAbs showed good binding activity in SHRV-infected epithelioma papulosum cyprini cells. In an indirect fluorescence assay, the MAbs gave varied staining patterns depending upon the viral structural proteins recognized.
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  • Kasornchandra, J., Caswell-Reno, P., Lannan, C. N., & Fryer, J. L. (1992, December). Development and Characterization of Monoclonal Antibodies against the Snakehead Rhabdovirus. Journal of Aquatic Animal Health, 4(4), 286-291.
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  • 4
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  • This publication is the result, in part, of research sponsored by Oregon Sea Grant with funds from the National Oceanic and Atmospheric Administration, Office of Sea Grant (grant NA 89AA-D-SG 108, project R/FSD-14) and from the U.S. Agency for International Development (project 7-276).
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