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Construction of a SNP and SSR linkage map in autotetraploid blueberry using genotyping by sequencing

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Abstract
  • The construction of the first genetic map in autotetraploid blueberry has been made possible by the development of new SNP markers developed using genotyping by sequencing in a mapping population created from a cross between two key highbush blueberry cultivars, Draper × Jewel (Vaccinium corymbosum). The novel SNP markers were supplemented with existing SSR markers to enable the alignment of parental maps. In total, 1794 single nucleotide polymorphic (SNP) markers and 233 simple sequence repeat (SSR) markers exhibited segregation patterns consistent with a random chromosomal segregation model for meiosis in an autotetraploid. Of these, 700 SNPs and 85 SSRs were utilized for construction of the ‘Draper’ genetic map, and 450 SNPs and 86 SSRs for the ‘Jewel’ map. The ‘Draper’ map comprises 12 linkage groups (LG), associated with the haploid chromosome number for blueberry, and totals 1621 cM while the ‘Jewel’ map comprises 20 linkage groups totalling 1610 cM. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents.
  • This is the publisher’s final pdf. The published article is copyrighted by Springer and can be found at: http://link.springer.com/journal/11032
  • Keywords: Vaccinium corymbosum, Tetraploid Map, Genotyping by sequencing, Autotetraploid
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  • McCallum, S., Graham, J., Jorgensen, L., Rowland, L. J., Bassil, N. V., Hancock, J. F., ... & Hackett, C. A. (2016). Construction of a SNP and SSR linkage map in autotetraploid blueberry using genotyping by sequencing. Molecular Breeding, 36(4), 41. doi:10.1007/s11032-016-0443-5
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  • 36
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  • 4
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  • This work was funded through Horticulture Link (HL0190), and all contributing partners are gratefully acknowledged. Project collaboration with the Specialty Crop Research Initiative-funded project (Grant 2008-51180-04861 entitled 'Generating genomic tools for blueberry improvement') has provided valuable access to plant material, genetic resources and advice. Support for this work from the Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS) is gratefully acknowledged
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