Lipidomics and H₂¹⁸O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos Public Deposited

http://ir.library.oregonstate.edu/concern/articles/j6731542t

This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Elsevier. The published article can be found at: http://www.sciencedirect.com/science/article/pii/S2213231716300064

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  • Lipidomics and (H2O)-O-18 labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in a-tocopherol deficient zebrafish (danio rerio) embryos
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  • We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without (E−) or with added α-tocopherol (E+, 500 mg RRR-α-tocopheryl acetate/kg diet) for a minimum of 80 days, and then spawned them to obtain E− and E+ embryos. The E− compared with E+ embryos were 82% less responsive (p<0.01) to a light/dark stimulus at 96 h post-fertilization (hpf), demonstrating impaired locomotor behavior, even in the absence of gross morphological defects. Evaluation of phospholipid (PL) and lysophospholipid (lyso-PL) composition using untargeted lipidomics in E− compared with E+ embryos at 24, 48, 72, and 120 hpf showed that four PLs and three lyso-PLs containing docosahexaenoic acid (DHA), including lysophosphatidylcholine (LPC 22:6, required for transport of DHA into the brain, p<0.001), were at lower concentrations in E− at all time-points. Additionally, H₂¹⁸O labeling experiments revealed enhanced turnover of LPC 22:6 (p<0.001) and three other DHA-containing PLs in the E− compared with the E+ embryos, suggesting that increased membrane remodeling is a result of PL depletion. Together, these data indicate that α-tocopherol deficiency in the zebrafish embryo causes the specific depletion and increased turnover of DHA-containing PL and lyso-PLs, which may compromise DHA delivery to the brain and thereby contribute to the functional impairments observed in E− embryos.
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  • McDougall, M. Q., Choi, J., Stevens, J. F., Truong, L., Tanguay, R. L. & Traber, M. G. (2016). Lipidomics and H₂¹⁸O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos. Redox Biology, 8, 165-174. doi:10.1016/j.redox.2016.01.004
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  • description.provenance : Made available in DSpace on 2016-07-14T19:23:38Z (GMT). No. of bitstreams: 3 license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5) McDougallLipidomicsandH2180OLabeling.pdf: 2975064 bytes, checksum: 74f3fc3ec344d5f62bb8a095262e02b9 (MD5) McDougallLipidomicsandH2180OLabelingSupplement.pdf: 61709 bytes, checksum: 2a8638135c1e9130b8a02cdeb786da10 (MD5) Previous issue date: 2016-08
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-07-14T19:23:06Z No. of bitstreams: 3 license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5) McDougallLipidomicsandH2180OLabeling.pdf: 2975064 bytes, checksum: 74f3fc3ec344d5f62bb8a095262e02b9 (MD5) McDougallLipidomicsandH2180OLabelingSupplement.pdf: 61709 bytes, checksum: 2a8638135c1e9130b8a02cdeb786da10 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-07-14T19:23:38Z (GMT) No. of bitstreams: 3 license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5) McDougallLipidomicsandH2180OLabeling.pdf: 2975064 bytes, checksum: 74f3fc3ec344d5f62bb8a095262e02b9 (MD5) McDougallLipidomicsandH2180OLabelingSupplement.pdf: 61709 bytes, checksum: 2a8638135c1e9130b8a02cdeb786da10 (MD5)

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