The kinetochore interaction network (KIN) of ascomycetes Public Deposited

http://ir.library.oregonstate.edu/concern/articles/jd472z261

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  • Chromosome segregation relies on coordinated activity of a large assembly of proteins, the kinetochore interaction network (KIN). How conserved the underlying mechanisms driving the epigenetic phenomenon of centromere and kinetochore assembly and maintenance are remains unclear, even though various eukaryotic models have been studied. More than 50 different proteins, many in multiple copies, comprise the KIN or are associated with fungal centromeres and kinetochores. Proteins isolated from immune sera recognized centromeric regions on chromosomes and thus were named centromere proteins (CENPs). CENP-A, sometimes called centromere-specific H3 (CenH3), is incorporated into nucleosomes within or near centromeres. The constitutive centromere-associated network (CCAN) assembles on this specialized chromatin, likely based on specific interactions with and requiring presence of CENP-C. The outer kinetochore comprises the Knl1-Mis12-Ndc80 (KMN) protein complexes that connect CCAN to spindles, accomplished by binding and stabilizing microtubules (MTs) and in the process generating load-bearing assemblies for chromatid segregation. In most fungi the Dam1/DASH complex connects the KMN complexes to MTs. Fungi present a rich resource to investigate mechanistic commonalities but also differences in kinetochore architecture. While ascomycetes have sets of CCAN and KMN proteins that are conserved with those of budding yeast or metazoans, searching other major branches of the fungal kingdom revealed that CCAN proteins are poorly conserved at the primary sequence level. Several conserved binding motifs or domains within KMN complexes have been described recently, and these features of ascomycete KIN proteins are shared with most metazoan proteins. In addition, several ascomycete-specific domains have been identified here.
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  • Freitag, M. (2016). The kinetochore interaction network (KIN) of ascomycetes. Mycologia, 108(3), 485-505. doi:10.3852/15-182
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  • description.provenance : Made available in DSpace on 2016-06-20T16:50:27Z (GMT). No. of bitstreams: 5 FreitagKinetochoreInteractionNetwork.pdf: 2554556 bytes, checksum: 70afaa4c2646f9f523bc5fc7a128f805 (MD5) FreitagKinetochoreInteractionNetworkSuppTableI.pdf: 33294 bytes, checksum: 2c94d2d7f5e730afbef2d52f7be77a4e (MD5) FreitagKinetochoreInteractionNetwork SuppTableII.pdf: 18173 bytes, checksum: 41068ec92c171612b5fe0199a29ce81b (MD5) FreitagKinetochoreInteractionNetworkSuppTableIII.pdf: 17519 bytes, checksum: b36dc26cd2681b4c9748cba1cb4bbba3 (MD5) FreitagKinetochoreInteractionNetworkSuppTableIV.pdf: 21666 bytes, checksum: 44bdff5c66aef04a00d5532fc36c981a (MD5) Previous issue date: 2016-06
  • description.provenance : Submitted by Patricia Black (patricia.black@oregonstate.edu) on 2016-06-20T16:50:11Z No. of bitstreams: 5 FreitagKinetochoreInteractionNetwork.pdf: 2554556 bytes, checksum: 70afaa4c2646f9f523bc5fc7a128f805 (MD5) FreitagKinetochoreInteractionNetworkSuppTableI.pdf: 33294 bytes, checksum: 2c94d2d7f5e730afbef2d52f7be77a4e (MD5) FreitagKinetochoreInteractionNetwork SuppTableII.pdf: 18173 bytes, checksum: 41068ec92c171612b5fe0199a29ce81b (MD5) FreitagKinetochoreInteractionNetworkSuppTableIII.pdf: 17519 bytes, checksum: b36dc26cd2681b4c9748cba1cb4bbba3 (MD5) FreitagKinetochoreInteractionNetworkSuppTableIV.pdf: 21666 bytes, checksum: 44bdff5c66aef04a00d5532fc36c981a (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-06-20T16:50:27Z (GMT) No. of bitstreams: 5 FreitagKinetochoreInteractionNetwork.pdf: 2554556 bytes, checksum: 70afaa4c2646f9f523bc5fc7a128f805 (MD5) FreitagKinetochoreInteractionNetworkSuppTableI.pdf: 33294 bytes, checksum: 2c94d2d7f5e730afbef2d52f7be77a4e (MD5) FreitagKinetochoreInteractionNetwork SuppTableII.pdf: 18173 bytes, checksum: 41068ec92c171612b5fe0199a29ce81b (MD5) FreitagKinetochoreInteractionNetworkSuppTableIII.pdf: 17519 bytes, checksum: b36dc26cd2681b4c9748cba1cb4bbba3 (MD5) FreitagKinetochoreInteractionNetworkSuppTableIV.pdf: 21666 bytes, checksum: 44bdff5c66aef04a00d5532fc36c981a (MD5)

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