Article
 

DNA repair and crossing over favor similar chromosome regions as discovered in radiation hybrid of Triticum

Public Deposited

Contenu téléchargeable

Télécharger le fichier PDF
https://ir.library.oregonstate.edu/concern/articles/k35695142

Descriptions

Attribute NameValues
Creator
Abstract
  • Background: The uneven distribution of recombination across the length of chromosomes results in inaccurate estimates of genetic to physical distances. In wheat (Triticum aestivum L.) chromosome 3B, it has been estimated that 90% of the cross over events occur in distal sub-telomeric regions representing 40% of the chromosome. Radiation hybrid (RH) mapping which does not rely on recombination is a strategy to map genomes and has been widely employed in animal species and more recently in some plants. RH maps have been proposed to provide i) higher and ii) more uniform resolution than genetic maps, and iii) to be independent of the distribution patterns observed for meiotic recombination. An in vivo RH panel was generated for mapping chromosome 3B of wheat in an attempt to provide a complete scaffold for this ~1 Gb segment of the genome and compare the resolution to previous genetic maps. Results: A high density RH map with 541 marker loci anchored to chromosome 3B spanning a total distance of 1871.9 cR was generated. Detailed comparisons with a genetic map of similar quality confirmed that i) the overall resolution of the RH map was 10.5 fold higher and ii) six fold more uniform. A significant interaction (r = 0.879 at p = 0.01) was observed between the DNA repair mechanism and the distribution of crossing-over events. This observation could be explained by accepting the possibility that the DNA repair mechanism in somatic cells is affected by the chromatin state in a way similar to the effect that chromatin state has on recombination frequencies in gametic cells. Conclusions: The RH data presented here support for the first time in vivo the hypothesis of non-casual interaction between recombination hot-spots and DNA repair. Further, two major hypotheses are presented on how chromatin compactness could affect the DNA repair mechanism. Since the initial RH application 37 years ago, we were able to show for the first time that the iii) third hypothesis of RH mapping might not be entirely correct.
  • Keywords: Physical mapping, Deletion mutant, Non homologous end joining, Wheat chromosome 3B, Chromatin\, , Radiation hybrid, Gamma radiation
Resource Type
DOI
Date Available
Date Issued
Citation
  • Ajay Kumar, Sachin Kumar, Aakash Goyal, Muhammad J Iqbal, Vijay K Tiwari, Munevver Dogramaci, . . . . (2012). DNA repair and crossing over favor similar chromosome regions as discovered in radiation hybrid of triticum. BMC Genomics, 13(1), 339-339. doi: 10.1186/1471-2164-13-339
Journal Title
Journal Volume
  • 13
Journal Issue/Number
  • 1
Academic Affiliation
Déclaration de droits
Funding Statement (additional comments about funding)
  • This work was supported by funding from the National Science Foundation, Plant Genome Research Program (NSF-PGRP) grant No. IOS-0822100 to SFK. Additional support from the NSF-PGRP Developing Country Collaboration aided the assistance of student interns A.Kumar, S.K., A.G., and V.T.; F.M.B was partially supported by Program Master and Back Regione Autonoma della Sardegna and Monsanto Beachell-Borlaug International Scholarship.
Publisher
Peer Reviewed
Language
Replaces

Des relations

Parents:

This work has no parents.

Articles

La vignette Titre Date de téléchargement Visibilité actes
file details TiwariVijayCropSoilScienceDNARepairCrossing.pdf 2017-07-10 Public Télécharger