Article
 

MHC Class I Antigen Presentation of DRiP-Derived Peptides from a Model Antigen Is Not Dependent on the AAA ATPase p97

Pubblico Deposited

Contenuto scaricabile

Scarica il pdf
https://ir.library.oregonstate.edu/concern/articles/kd17ct655

Descriptions

Attribute NameValues
Creator
Abstract
  • CD8⁺ T cells are responsible for killing cells of the body that have become infected or oncogenically transformed. In order to do so, effector CD8⁺ T cells must recognize their cognate antigenic peptide bound to a MHC class I molecule that has been directly presented by the target cell. Due to the rapid nature of antigen presentation, it is believed that antigenic peptides are derived from a subset of newly synthesized proteins which are degraded almost immediately following synthesis and termed Defective Ribosomal Products or DRiPs. We have recently reported on a bioassay which can distinguish antigen presentation of DRiP substrates from other forms of rapidly degraded proteins and found that poly-ubiquitin chain disassembly may be necessary for efficient DRiP presentation. The AAA ATPase p97 protein is necessary for efficient crosspresentation of antigens on MHC class I molecules and plays an important role in extracting mis-folded proteins from the endoplasmic reticulum. Here, we find that genetic ablation or chemical inhibition of p97 does not diminish DRiP antigen presentation to any great extent nor does it alter the levels of MHC class I molecules on the cell surface, despite our observations that p97 inhibition increased the levels of poly-ubiquitinated proteins in the cell. These data demonstrate that inhibiting poly-ubiquitin chain disassembly alone is insufficient to abolish DRiP presentation.
License
Resource Type
DOI
Date Available
Date Issued
Citation
Journal Title
Journal Volume
  • 8
Journal Issue/Number
  • 7
Academic Affiliation
Dichiarazione dei diritti
Funding Statement (additional comments about funding)
  • Funding was provided by NIH grant 1K22AI089861 - 01A1 (www.nih.gov). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher
Peer Reviewed
Language
Replaces

Le relazioni

Parents:

This work has no parents.

Elementi

Thumbnail Titolo Data caricata Visibilità Azioni
file details PalmerAmyLBiomedicalSciencesMHCClassAntigen.pdf 2017-07-13 Pubblico Scaricare