The Fusarium graminearum Histone H3 K27 Methyltransferase KMT6 Regulates Development and Expression of Secondary Metabolite Gene Clusters Public Deposited

http://ir.library.oregonstate.edu/concern/articles/kh04dr411

This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by the Public Library of Science. The published article can be found at:  http://www.plosgenetics.org/.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • The cereal pathogen Fusarium graminearum produces secondary metabolites toxic to humans and animals, yet coordinated transcriptional regulation of gene clusters remains largely a mystery. By chromatin immunoprecipitation and high-throughput DNA sequencing (ChIP-seq) we found that regions with secondary metabolite clusters are enriched for trimethylated histone H3 lysine 27 (H3K27me3), a histone modification associated with gene silencing. H3K27me3 was found predominantly in regions that lack synteny with other Fusarium species, generally subtelomeric regions. Di- or trimethylated H3K4 (H3K4me2/3), two modifications associated with gene activity, and H3K27me3 are predominantly found in mutually exclusive regions of the genome. To find functions for H3K27me3, we deleted the gene for the putative H3K27 methyltransferase, KMT6, a homolog of Drosophila Enhancer of zeste, E(z). The kmt6 mutant lacks H3K27me3, as shown by western blot and ChIP-seq, displays growth defects, is sterile, and constitutively expresses genes for mycotoxins, pigments and other secondary metabolites. Transcriptome analyses showed that 75% of 4,449 silent genes are enriched for H3K27me3. A subset of genes that were enriched for H3K27me3 in WT gained H3K4me2/3 in kmt6. A largely overlapping set of genes showed increased expression in kmt6. Almost 95% of the remaining 2,720 annotated silent genes showed no enrichment for either H3K27me3 or H3K4me2/3 in kmt6. In these cases mere absence of H3K27me3 was insufficient for expression, which suggests that additional changes are required to activate genes. Taken together, we show that absence of H3K27me3 allowed expression of an additional 14% of the genome, resulting in derepression of genes predominantly involved in secondary metabolite pathways and other species-specific functions, including putative secreted pathogenicity factors. Results from this study provide the framework for novel targeted strategies to control the ‘‘cryptic genome’’, specifically secondary metabolite expression.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Connolly LR, Smith KM, Freitag M (2013) The Fusarium graminearum Histone H3 K27 Methyltransferase KMT6 Regulates Development and Expression of Secondary Metabolite Gene Clusters. PLoS Genetics 9(10): e1003916. doi:10.1371/journal.pgen.1003916
Series
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-04-01T19:57:22Z No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ConnollyLanelleBiochemistryBiophysicsFusariumGraminearumHistone.pdf: 9613330 bytes, checksum: 1cd2520a5979dc3222e31743cc4b013e (MD5) ConnollyLanelleBiochemistryBiophysicsFusariumGraminearumHistone_SupportingInformation.zip: 9294822 bytes, checksum: 1d562b1e0167a0809d35946b68a63109 (MD5)
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-04-01T19:57:37Z (GMT) No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ConnollyLanelleBiochemistryBiophysicsFusariumGraminearumHistone.pdf: 9613330 bytes, checksum: 1cd2520a5979dc3222e31743cc4b013e (MD5) ConnollyLanelleBiochemistryBiophysicsFusariumGraminearumHistone_SupportingInformation.zip: 9294822 bytes, checksum: 1d562b1e0167a0809d35946b68a63109 (MD5)
  • description.provenance : Made available in DSpace on 2014-04-01T19:57:37Z (GMT). No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) ConnollyLanelleBiochemistryBiophysicsFusariumGraminearumHistone.pdf: 9613330 bytes, checksum: 1cd2520a5979dc3222e31743cc4b013e (MD5) ConnollyLanelleBiochemistryBiophysicsFusariumGraminearumHistone_SupportingInformation.zip: 9294822 bytes, checksum: 1d562b1e0167a0809d35946b68a63109 (MD5) Previous issue date: 2013-10-31

Relationships

In Administrative Set:
Last modified: 07/26/2017 Default
Citations:

EndNote | Zotero | Mendeley