Novel Nitro-PAH Formation from Heterogeneous Reactions of PAHs with NO₂, NO₃/N₂O₅, and OH Radicals: Prediction, Laboratory Studies and Mutagenicity Public Deposited


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Alternative Title
  • The heterogeneous reactions of benzo[a]pyrene-d₁₂ (BaP-d₁₂), benzo[k]fluoranthene-d₁₂ (BkF-d₁₂), benzo[ghi]perylene-d₁₂ (BghiP-d₁₂), dibenzo[a,i]pyrene-d₁₄ (DaiP-d₁₄), and dibenzo[a,l]pyrene (DalP) with NO₂, NO₃/N₂O₅, and OH radicals were investigated at room temperature and atmospheric pressure in an indoor Teflon chamber and novel mono NO₂-DaiP, and mono NO₂-DalP products were identified. Quartz fiber filters (QFF) were used as a reaction surface and the filter extracts were analyzed by GC/MS for nitrated-PAHs (NPAHs) and tested in the Salmonella mutagenicity assay, using Salmonella typhimurium strain TA98 (with and without metabolic activation). In parallel to the laboratory experiments, a theoretical study was conducted to rationalize the formation of NPAH isomers based on the thermodynamic stability of OH-PAH intermediates, formed from OH-radical-initiated reactions. NO₂ and NO₃/N₂O₅ were effective oxidizing agents in transforming PAHs to NPAHs, with BaP-d₁₂ being the most readily nitrated. Reaction of BaP-d₁₂, BkF-d₁₂ and BghiP-d₁₂ with NO₂ and NO₃/N₂O₅ resulted in the formation of more than one mono-nitro isomer product, while the reaction of DaiP-d₁₄ and DalP resulted in the formation of only one mono-nitro isomer product. The direct-acting mutagenicity increased the most after NO₃/N₂O₅ exposure, particularly for BkF-d₁₂ in which di-NO₂-BkF-d₁₀ isomers were measured. The deuterium isotope effect study suggested that substitution of deuterium for hydrogen lowered both the direct and indirect acting mutagenicity of NPAHs and may result in an underestimation of the mutagenicity of the novel NPAHs identified in this study.
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  • Jariyasopit, N., McIntosh, M., Zimmermann, K., Arey, J., Atkinson, R., Cheong, P. H. Y., ... & Massey Simonich, S. L. (2014). Novel Nitro-PAH Formation from Heterogeneous Reactions of PAHs with NO₂, NO₃/N₂O₅, and OH Radicals: Prediction, Laboratory Studies, and Mutagenicity. Environmental Science & Technology, 48(1), 412-419. doi:10.1021/es4043808
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Journal Volume
  • 48
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  • 1
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Funding Statement (additional comments about funding)
  • This publication was made possible in part by grant number P30ES00210 from the National Institute of Environmental Health Sciences (NIEHS), NIH and NIEHS Grant P42 ES016465, and the U.S. National Science Foundation (ATM-0841165).
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