Identification of Mycobacterium avium genes associated with resistance to host antimicrobial peptides Public Deposited

http://ir.library.oregonstate.edu/concern/articles/mc87pw19q

This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Author(s) and published by the Society for General Microbiology. It can be found at:  http://jmm.sgmjournals.org/

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Antimicrobial peptides are an important component of the innate immune defense. Mycobacterium avium subsp hominissuis (M. avium) is an organism that establishes contact with the respiratory and gastrointestinal mucosa as a necessary step for infection. M. avium is resistant to high concentrations of polymyxin B, a surrogate for antimicrobial peptides. To determine gene-encoding proteins that are associated with this resistance, we screened a transposon library of M. avium strain 104 for susceptibility to polymyxin B. Ten susceptible mutants were identified and the inactivated genes sequenced. The greatest majority of the genes were related to cell wall synthesis and permeability. The mutants were then examined for their ability to enter macrophages and to survive macrophage killing. Three clones among the mutants had impaired uptake by macrophages compared to the wild-type strain, and all ten clones were attenuated in macrophages. The mutants were shown also to be susceptible to cathelicidin (LL-37), in contrast to the wild-type bacterium. All but one of the mutants were significantly attenuated in mice. In conclusion, this study indicated that the M. avium envelope is the primary defense against host antimicrobial peptides.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Motamedi, N., Danelishvili, L., & Bermudez, L. E. (2014). Identification of Mycobacterium avium genes associated with resistance to host antimicrobial peptides. Journal of Medical Microbiology, 63(Pt 7), 923-930. doi:10.1099/jmm.0.072744-0
Series
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces

Relationships

In Administrative Set:
Last modified: 10/27/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items