Genome-Wide Characterization of Light-Regulated Genes in Neurospora crassa Public Deposited

http://ir.library.oregonstate.edu/concern/articles/n583xw60q

This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by the Genetics Society of America. The published article can be found at:  http://g3journal.org/.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • The filamentous fungus Neurospora crassa responds to light in complex ways. To thoroughly study the transcriptional response of this organism to light, RNA-seq was used to analyze capped and polyadenylated mRNA prepared from mycelium grown for 24 hr in the dark and then exposed to light for 0 (control) 15, 60, 120, and 240 min. More than three-quarters of all defined protein coding genes (79%) were expressed in these cells. The increased sensitivity of RNA-seq compared with previous microarray studies revealed that the RNA levels for 31% of expressed genes were affected two-fold or more by exposure to light. Additionally, a large class of mRNAs, enriched for transcripts specifying products involved in rRNA metabolism, showed decreased expression in response to light, indicating a heretofore undocumented effect of light on this pathway. Based on measured changes in mRNA levels, light generally increases cellular metabolism and at the same time causes significant oxidative stress to the organism. To deal with this stress, protective photopigments are made, antioxidants are produced, and genes involved in ribosome biogenesis are transiently repressed.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Wu, C., Yang, F., Smith, K. M., Peterson, M., Dekhang, R., Zhang, Y., ... & Sachs, M. S. (2014). Genome-wide characterization of light-regulated genes in Neurospora crassa. G3: Genes Genomes Genetics, 4(9), 1731-1745. doi:10.1534/g3.114.012617
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-11-03T21:14:58Z No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) SmithKristinaBiochemBiophysGenome-WideCharacterization.pdf: 3071661 bytes, checksum: 33440195e2aad167bd00b29dd80079f8 (MD5) SmithKristinaBiochemBiophysGenome-WideCharacterization_SupportingInformation.zip: 12488420 bytes, checksum: ca5a813450215ab5fbf77bfafb9bbcb5 (MD5)
  • description.provenance : Made available in DSpace on 2014-11-03T21:15:14Z (GMT). No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) SmithKristinaBiochemBiophysGenome-WideCharacterization.pdf: 3071661 bytes, checksum: 33440195e2aad167bd00b29dd80079f8 (MD5) SmithKristinaBiochemBiophysGenome-WideCharacterization_SupportingInformation.zip: 12488420 bytes, checksum: ca5a813450215ab5fbf77bfafb9bbcb5 (MD5) Previous issue date: 2014-09-01
  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-11-03T21:15:14Z (GMT) No. of bitstreams: 3 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) SmithKristinaBiochemBiophysGenome-WideCharacterization.pdf: 3071661 bytes, checksum: 33440195e2aad167bd00b29dd80079f8 (MD5) SmithKristinaBiochemBiophysGenome-WideCharacterization_SupportingInformation.zip: 12488420 bytes, checksum: ca5a813450215ab5fbf77bfafb9bbcb5 (MD5)

Relationships

Parents:

This work has no parents.

Last modified Default

Items