Article
 

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/articles/n870zr42t

Descriptions

Attribute NameValues
Creator
Abstract
  • The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction of desired traits into grapevine without heritable modifications to the genome. This vector expresses recombinant proteins in the phloem tissue that is involved in sugar transport throughout the plant, from leaves to roots to berries. Furthermore, the vector provides a powerful RNA interference (RNAi) capability of regulating the expression of endogenous genes via virus-induced gene-silencing (VIGS) technology. Additional advantages of this vector include superb genetic capacity and stability, as well as the swiftness of technology implementation. The most significant applications of the viral vector include functional genomics of the grapevine and disease control via RNAi-enabled vaccination against pathogens or invertebrate pests.
  • This is the publisher’s final pdf. The published article is copyrighted by the American Society for Microbiology and can be found at: http://jvi.asm.org/. The authors have paid a fee to allow immediate free access to this article.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Kurth, E. G., Peremyslov, V. V., Prokhnevsky, A. I., Kasschau, K. D., Miller, M., Carrington, J. C., & Dolja, V. V. (2012). Virus-derived gene expression and RNA interference vector for grapevine. Journal of Virology, 86(11), 6002-6009. doi: 10.1128/JVI.00436-12
Journal Title
Journal Volume
  • 86
Journal Issue/Number
  • 11
Academic Affiliation
Rights Statement
Funding Statement (additional comments about funding)
  • This work was supported by contract 2010-744 from Vinoculate, Inc., Soledad, CA, a subcontract from USDA-NIFA (award no. 2009-04401), and BARD award no. IS-4314-10C to V.V.D. The work in J.C.C. lab is supported by NSF award MCB-1231726 and NIH award AI43288.
Publisher
Peer Reviewed
Language
Replaces

Relationships

Parents:

This work has no parents.

Items