Application of marker-assisted selection and genome-wide association scanning to the development of winter food barley germplasm resources Public Deposited

http://ir.library.oregonstate.edu/concern/articles/n870zs820

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  • Barley (Hordeum vulgare) is an important component of heart-healthy whole grain diets because it contains β-glucan. All current US barley varieties with high β-glucan are spring habit and have waxy starch. Winter varieties have agronomic advantages but require low-temperature tolerance (LTT). Vernalization sensitivity (VS) is associated with higher levels of LTT. To rapidly develop fall-sown varieties with LTT and higher grain β-glucan, we therefore used marker-assisted selection (MAS) at the WX and VRN-H2 loci. The MAS-derived lines, together with unrelated non-waxy germplasm developed via phenotypic selection (PS), were used for a genome-wide association scan (GWAS). The panel was phenotyped for grain β-glucan, LTT and VS. It was genotyped with 3072 single-nucleotide polymorphisms (SNPs) and allele-specific primers. Marker-assisted selection fixed target alleles at both loci but only one of the target phenotypes (higher β-glucan percentage) was achieved. Variation for VS and LTT is attributable to (i) incomplete information about VRN-H1 at the outset of the project and (ii) unexpected allelic variation at VRN-H3 with a large effect on VS and LTT.
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  • Chutimanitsakun, Y., Cuesta‐Marcos, A., Chao, S., Corey, A., Filichkin, T., Fisk, S., ... & Hayes, P. M. (2013). Application of marker‐assisted selection and genome‐wide association scanning to the development of winter food barley germplasm resources. Plant Breeding, 132(6), 563-570. doi:10.1111/pbr.12086
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  • 132
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  • 6
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  • description.provenance : Approved for entry into archive by Erin Clark(erin.clark@oregonstate.edu) on 2014-03-04T21:04:28Z (GMT) No. of bitstreams: 1 ChutimanitsakunYadaCropSoilScienceApplicationMarker-Assisted.pdf: 1100012 bytes, checksum: 9d04f3a04a669c1bc363cb2788e1868b (MD5)
  • description.provenance : Made available in DSpace on 2014-03-04T21:04:28Z (GMT). No. of bitstreams: 1 ChutimanitsakunYadaCropSoilScienceApplicationMarker-Assisted.pdf: 1100012 bytes, checksum: 9d04f3a04a669c1bc363cb2788e1868b (MD5) Previous issue date: 2013-12
  • description.provenance : Submitted by Erin Clark (erin.clark@oregonstate.edu) on 2014-03-04T16:48:09Z No. of bitstreams: 1 ChutimanitsakunYadaCropSoilScienceApplicationMarker-Assisted.pdf: 1100012 bytes, checksum: 9d04f3a04a669c1bc363cb2788e1868b (MD5)

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