Expression of serotonin, chromogranin-A, serotonin receptor-2B, tryptophan hydroxylase-1, and serotonin reuptake transporter in the intestine of dogs with chronic enteropathy Public Deposited

http://ir.library.oregonstate.edu/concern/articles/nc580p18m

This is an author's peer-reviewed final manuscript, as accepted by the publisher. The article is in press and will be published in the Journal of Veterinary Diagnostic Investigation.

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  • Serotonin regulates many intestinal motor and sensory functions. Altered serotonergic metabolism has been described in human gastrointestinal diseases. The objective of our study was to compare expression of several components of the serotonergic system [serotonin (5-HT), serotonin reuptake transporter protein (SERT), tryptophan hydroxylase-1 (TPH-1), 5-HT receptor[subscript 2B] (5-HT[subscript 2B])] and the enterochromaffin cell marker chromogranin-A (CgA) in the intestinal mucosa between dogs with chronic enteropathy and healthy controls. Serotonin and CgA expression were determined by immunohistochemistry using banked and prospectively obtained, paraffin-embedded canine gastrointestinal biopsies (n = 11), and compared to a control group of canine small intestinal sections (n = 10). Expression of SERT, TPH-1, and 5-HT[subscript 2B] were determined via real-time reverse transcription polymerase chain reaction (qRT-PCR) using prospectively collected endoscopic duodenal biopsies (n = 10) and compared to an additional control group of control duodenal biopsies (n = 8, Control Group 2) showing no evidence of intestinal inflammation. Dogs with chronic enteropathies showed strong staining for both 5-HT and CgA. Mean positive cells per high power field (HPF) were significantly increased for both compounds in dogs with chronic enteropathies (p < 0.001 for 5-HT and p < 0.05 CgA). The number of 5-HT–positive and CgA-positive cells/HPF showed significant correlation in the entire group of dogs, including both diseased and healthy individuals (Pearson r² = 0.2433, p = 0.016). No significant differences were observed for SERT, TPH-1, or 5-HT[subscript 2B] expression; however, dogs with chronic enteropathy showed greater variability in expression of TPH-1 and 5-HT[subscript 2B]. We conclude that components of the neuroendocrine system show altered expression in the intestinal mucosa of dogs with chronic enteropathy. These changes may contribute to nociception and clinical signs in these patients.
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